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quick update
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y9c committed Nov 8, 2024
1 parent dd8182c commit 20a4f3c
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2 changes: 2 additions & 0 deletions cutseq/run.py
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Expand Up @@ -243,6 +243,8 @@ def __init__(self):
"ECLIP6": "ACACGACGCTCTTCCGATCTXX<XNNNNNNAGATCGGAAGAGCACACGTC",
# eCLIP, SAC-seq, cDNA ligation method, with 10 nt UMI
"ECLIP10": "ACACGACGCTCTTCCGATCTXX<XNNNNNNNNNNAGATCGGAAGAGCACACGTC",
# cDNA88, cDNA ligation method, with 8 nt UMI (left) and 8 nt UMI (right)
"SACSEQV3": "AGTTCTACAGTCCGACGATCTNNNNNNNNX>XXNNNNNNNNAGATCGGAAGAGCACACGTC",
# p5 - [might be 6bp of polyC] - reverse insert (cDNA) - adaptase tail (CCCCCC) - p7
# 6nt of polyG in 5' of R1 might from random RT primer
# adaptase tail can be as long as 15bp at the 5' of R2 of polyG)
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2 changes: 1 addition & 1 deletion pyproject.toml
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@@ -1,6 +1,6 @@
[tool.poetry]
name = "cutseq"
version = "0.0.54"
version = "0.0.55"
description = "Automatically cut adapter / barcode / UMI from NGS data"
authors = ["Ye Chang <[email protected]>"]
license = "MIT"
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