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update readme and links in tutos
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dbdimitrov committed Mar 7, 2024
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28 changes: 1 addition & 27 deletions README.md
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Expand Up @@ -17,33 +17,7 @@ LIANA+ is a scalable framework that integrates and extends existing methods and
We welcome suggestions, ideas, and contributions! Please use do not hesitate to contact us, or use the issues or the [LIANA+ Development project](https://github.com/orgs/saezlab/projects/16) to make suggestions.

## Tutorials

### Single-cell/Dissociated Data

- [LIANA's basic tutorial](https://liana-py.readthedocs.io/en/latest/notebooks/basic_usage.html) in dissociated single-cell data

#### Multi-condition

- [Differential Expression Analysis for CCC with PyDeSeq2](https://liana-py.readthedocs.io/en/latest/notebooks/targeted.html) that also shows the inference of causal **intracellular** signalling networks, downstream of CCC events.

- [Multicellular programmes with MOFA](https://liana-py.readthedocs.io/en/latest/notebooks/mofacellular.html). Using MOFA to obtain coordinates gene expression programmes across samples and conditions, as done in [Ramirez et al., 2023](https://europepmc.org/article/ppr/ppr620471).

- [LIANA with MOFA](https://liana-py.readthedocs.io/en/latest/notebooks/mofatalk.html). Using MOFA to infer intercellular communication programmes across samples and conditions, as initially proposed by Tensor-cell2cell.

- [LIANA with Tensor-cell2cell](https://liana-py.readthedocs.io/en/latest/notebooks/liana_c2c.html) to extract intercellular communication programmes across samples and conditions. Extensive tutorials combining LIANA & [Tensor-cell2cell](https://www.nature.com/articles/s41467-022-31369-2) are available [here](https://ccc-protocols.readthedocs.io/en/latest/index.html).


### Spatial Data

- [Learn spatially-informed relationships with MISTy](https://liana-py.readthedocs.io/en/latest/notebooks/misty.html) across (multi-) views.

- [Estimate local spatially-informed bivariate metrics](https://liana-py.readthedocs.io/en/latest/notebooks/bivariate.html). This tutorial shows how to estimate local spatially-informed bivariate metrics, such as the spatially-informed Pearson correlation coefficient or Cosine similarity.


### Others

- We also refer users to the [Cell-cell communication chapter](https://www.sc-best-practices.org/mechanisms/cell_cell_communication.html) in the [best-practices guide from Theis lab](https://www.nature.com/articles/s41576-023-00586-w). There we provide an overview of the common limitations and assumptions in CCC inference from (dissociated single-cell) transcriptomics data.

A set of extensive tutorials can be found in the [LIANA+ documentation](https://liana-py.readthedocs.io/en/latest/).

## API
For further information please check LIANA's [API documentation](https://liana-py.readthedocs.io/en/latest/api.html).
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3 changes: 1 addition & 2 deletions docs/source/index.rst
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Expand Up @@ -34,6 +34,7 @@ spatially-resolved, and multi-modal omics data.
api
release_notes
reference
notebooks/prior_knowledge

.. toctree::
:maxdepth: 1
Expand All @@ -42,8 +43,6 @@ spatially-resolved, and multi-modal omics data.

notebooks/basic_usage

notebooks/prior_knowledge

notebooks/liana_c2c

notebooks/mofacellular
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7 changes: 7 additions & 0 deletions docs/source/notebooks/basic_usage.ipynb
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"In this notebook we showcase how to use liana in its most basic form with toy data."
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"We also refer users to the [Cell-cell communication chapter](https://www.sc-best-practices.org/mechanisms/cell_cell_communication.html) in the [best-practices guide from Theis lab](https://www.nature.com/articles/s41576-023-00586-w). There we provide an overview of the common limitations and assumptions in CCC inference from (dissociated single-cell) transcriptomics data."
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4 changes: 3 additions & 1 deletion docs/source/notebooks/liana_c2c.ipynb
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"The power of [Tensor-cell2cell](https://github.com/earmingol/cell2cell) is in its ability to decompose latent patterns of intercellular communication in an untargeted manner, in theory being able to handle cell-cell communication results coming from any experimental design, regardless of its complexity.\n",
"\n",
"Simply put, tensor_cell2cell uses LIANA’s output by sample to build a 4D tensor, represented by 1) contexts, 2) interactions, 3) sender, and 4) receiver cell types. This tensor is then decomposed into a set of factors, which can be interpreted as low-dimensionality latent variables (vectors) that capture the CCC patterns across contexts.\n",
"We will combine LIANA with tensor_cell2cell to decipher potential ligand-receptor interaction changes."
"We will combine LIANA with tensor_cell2cell to decipher potential ligand-receptor interaction changes.\n",
"\n",
"Extensive tutorials combining LIANA & [Tensor-cell2cell](https://www.nature.com/articles/s41467-022-31369-2) are available [here](https://ccc-protocols.readthedocs.io/en/latest/index.html)."
]
},
{
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