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Annotating "N's" #480

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Glfrey opened this issue Dec 14, 2023 · 0 comments
Open

Annotating "N's" #480

Glfrey opened this issue Dec 14, 2023 · 0 comments

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@Glfrey
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Glfrey commented Dec 14, 2023

Hello again,

I'm just doing some experimental work with a genome which features some "N" sequences. When I query the annotation, the k-mers with "N's" in them are not returned with any hits. For example if the sequence is "NCCCCCCCGGCGGCCACTCCTCCTCCCACCGGCGGCT", the k-mer "NCCCCCCCGGCGGCCACTCCT" doesn't return any hits but the rest of the sequences k-mers do. I've confirmed this sequence exists in the genome and so should have been annotated.

Is this expected behavior I need to work with or something erroneous?

I'm currently using the Python API.

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