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2.8.1
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mikolmogorov committed Sep 2, 2020
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7 changes: 5 additions & 2 deletions README.md
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Expand Up @@ -3,7 +3,7 @@ Flye assembler

[![BioConda Install](https://img.shields.io/conda/dn/bioconda/flye.svg?style=flag&label=BioConda%20install)](https://anaconda.org/bioconda/flye)

### Version: 2.8
### Version: 2.8.1

Flye is a de novo assembler for single molecule sequencing reads,
such as those produced by PacBio and Oxford Nanopore Technologies.
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Latest updates
--------------

### Flye 2.8.1 release (02 Sep 2020)
* Added a new option `--hifi-error` to control the expected error rate of HiFi reads (no other changes)

### Flye 2.8 release (04 Aug 2020)
* Improvements in contiguity and speed for PacBio HiFi mode
* Using the `--meta` k-mer selection strategy in isolate assemblies as well.
Expand Down Expand Up @@ -128,7 +131,7 @@ The assemblies generated using Flye 2.8 could be downloaded from [Zenodo](https:
All datasets were run with default parameters for the corresponding read type
with the following exceptions: CHM13 T2T was run with `--min-overlap 10000 --asm-coverage 50`;
CHM1 was run with `--asm-coverage 50`. CHM13 HiFi and HG002 HiFi datasets were run in
`--pacbio-hifi` mode and reduced error rate threshold (0.003%).
`--pacbio-hifi` mode and `--hifi-error 0.003`.

Third-party
-----------
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4 changes: 4 additions & 0 deletions docs/NEWS.md
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Flye 2.8.1 release (02 Sep 2020)
===============================
* Added a new option `--hifi-error` to control the expected error rate of HiFi reads (no other changes)

Flye 2.8 release (04 Aug 2020)
==============================
* Improvements in contiguity and speed for PacBio HiFi mode
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8 changes: 6 additions & 2 deletions docs/USAGE.md
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Expand Up @@ -54,6 +54,8 @@ optional arguments:
minimum overlap between reads [auto]
--asm-coverage int reduced coverage for initial disjointig assembly [not
set]
--hifi-error float expected HiFi reads error rate (e.g. 0.01 or 0.001)
[0.01]
--plasmids rescue short unassembled plasmids
--meta metagenome / uneven coverage mode
--keep-haplotypes do not collapse alternative haplotypes
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### PacBio HiFi

Flye now supports assembly of PacBio HiFi protocol via `--pacbio-hifi` option.
The expected read error is <1%.
The expected read error is 1% by default. In case the reads are more accurate,
you can adjust `--hifi-error` parameter (for example to 0.001) to potentially
generate more complete assemblies.

### PacBio CLR

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All datasets were run with default parameters for the corresponding read type
with the following exceptions: CHM13 T2T was run with `--min-overlap 10000 --asm-coverage 50`;
CHM1 was run with `--asm-coverage 50`. CHM13 HiFi and HG002 HiFi datasets were run in
`--pacbio-hifi` mode and reduced error rate threshold (0.003%).
`--pacbio-hifi` mode and `--hifi-error 0.003`.

## <a name="algorithm"></a> Algorithm Description

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2 changes: 1 addition & 1 deletion flye/__version__.py
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__version__ = "2.8"
__version__ = "2.8.1"

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