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avikdatta committed Sep 20, 2023
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Showing 1 changed file with 13 additions and 21 deletions.
34 changes: 13 additions & 21 deletions analysis_pipelines.md
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<td><a href="https://nanostring.com/products/geomx-digital-spatial-profiler/geomx-dsp-overview/">geomxngspipeline</a></td>
<td>Active</td>
</tr>
<tr>
<td>ChIP-Seq alignment, peak calling and QC</td>
<td><a href="#nf-core_chipseq">nf-core/chipseq</a></td>
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<td><a href="#nf-core_ampliseq">nf-core/ampliseq</a></td>
<td>Untested</td>
</tr>
</tbody>
</table>
</div>
-->

<h2 id="snakemake-workflows_rna-seq-star-deseq2">Snakemake RNA-Seq workflow</h2>

<div>
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<tr>
<td style="border:hidden; width:25%"><b>Required inputs:</b></td>
<td style="border:hidden;">
<p>
We need following details to configure and run this pipeline:
<p>We need following details to configure and run this pipeline:</p>
<ul>
<li>List of sample IGF ids</li>
<li>Reference genome to use from Ensembl(e.g. Homo sapiens)</li>
<li>Sample groups for DESeq2 analysis</li>
</ul>
</p>
<details>
<summary>Click here for more information</summary><p/>
<p><b>Reference genome</b></p>
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<li>Genome build tag (e.g., GRCh38)</li>
</ul>
<p><b>Sample metadata</b></p>
Simple metadata:<pre>sample_id,condition
IGF001,untreated
IGF002,treated</pre>
Complex metadata:<pre>
sample_id,treatment_1,treatment_2
Simple metadata:<pre> sample_id,condition
IGF001,untreated
IGF002,treated</pre>
Complex metadata:<pre> sample_id,treatment_1,treatment_2
IGF001,untreated,untreated
IGF002,untreated,treated
IGF003,untreated,treated</pre>
<p><b>Sample group info</b></p>
Simple group:
Check this <a href="https://github.com/snakemake-workflows/rna-seq-star-deseq2/blob/master/.test/config_basic/config.yaml">example</a>
<pre><code>
Group: treated-vs-untreated
variable_of_interest: condition
level_of_interest: treated
</pre></code>
Complex group:
Check this <a href="https://github.com/snakemake-workflows/rna-seq-star-deseq2/blob/master/.test/config_complex/config.yaml">example</a>
<pre>Group: treatment_1_alone
Simple group: Check this <a href="https://github.com/snakemake-workflows/rna-seq-star-deseq2/blob/master/.test/config_basic/config.yaml">example</a>
<pre> Group: treated-vs-untreated
variable_of_interest: condition
level_of_interest: treated</pre>
Complex group: Check this <a href="https://github.com/snakemake-workflows/rna-seq-star-deseq2/blob/master/.test/config_complex/config.yaml">example</a>
<pre> Group: treatment_1_alone
variable_of_interest: treatment_1
level_of_interest: treated</pre>
Slevel_of_interest: treated</pre>
</details></td>
</tr>
<tr>
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