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Create a Cell-Free device collection #110

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vinoo-igem opened this issue Sep 20, 2021 · 4 comments · May be fixed by #220
Open

Create a Cell-Free device collection #110

vinoo-igem opened this issue Sep 20, 2021 · 4 comments · May be fixed by #220
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help wanted Extra attention is needed package request Suggestions for packages to develop or improve

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@vinoo-igem
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Yorgo, an iGEM Safety & Security intern did a deep dive into past use of cell-free systems in iGEM. I'll chat with him more, and hope to create a list of useful devices that have worked in the past, as well as essential parts/plasmids for a cell-free collection.
@vinoo-igem vinoo-igem added the package request Suggestions for packages to develop or improve label Sep 20, 2021
@vinoo-igem vinoo-igem self-assigned this Sep 20, 2021
@traci-igem traci-igem mentioned this issue Sep 22, 2021
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@jakebeal jakebeal added the help wanted Extra attention is needed label Oct 20, 2021
@yorgomoubayed
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Happy to contribute to the cell-free addition. The registry has a lot of interesting cell-free optimized parts; I've already recovered them during my fellowship. I can compile them and look into making a proposal!

@yorgomoubayed
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In 2017, the Lethbridge team developed a standardized, modular, and open-source ex vivo TX-TL system: http://parts.igem.org/Collections/Cell-Free_TX-TL

@yorgomoubayed
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In 2019, the Stanford team developed ourTXTL, a measurement standard for characterizing the relative strength of promoters in cell-free. They also implemented protocols for creating and lyophilizing cell-free lysis from E. coli and V. natriegens.

@yorgomoubayed
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A functional nucleic acids collection curated by the After iGEM Academia & Research Committee

@nickdelkis nickdelkis linked a pull request Feb 10, 2022 that will close this issue
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Create cell-free parts package iGEM-Engineering/iGEM-distribution
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@jakebeal @yorgomoubayed @vinoo-igem