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# import argparse | ||
# import logging | ||
# import os | ||
# import sys | ||
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# import numpy as np | ||
# import pandas as pd | ||
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# """initialize""" | ||
# pd.set_option("max_colwidth", 60) # column最大宽度 | ||
# pd.set_option("display.width", 150) # dataframe宽度 | ||
# pd.set_option("display.max_columns", None) # column最大显示数 | ||
# pd.set_option("display.max_rows", 100) # row最大显示数 | ||
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# dt_type = { | ||
# "chr_name": str, | ||
# "chr_index": str, | ||
# "ref_base": str, | ||
# "A": int, | ||
# "G": int, | ||
# "C": int, | ||
# "T": int, | ||
# "del_count": int, | ||
# "insert_count": int, | ||
# "ambiguous_count": int, | ||
# "deletion": str, | ||
# "insertion": str, | ||
# "ambiguous": str, | ||
# "mut_num": int, | ||
# } | ||
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# def logging_setting(): | ||
# # log setting | ||
# logging.basicConfig( | ||
# level=logging.DEBUG, | ||
# format="%(levelname)-5s @ %(asctime)s: %(message)s ", | ||
# datefmt="%Y-%m-%d %H:%M:%S", | ||
# stream=sys.stderr, | ||
# filemode="w", | ||
# force=True, | ||
# ) | ||
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# def argparse_setting(): | ||
# # argparse setting | ||
# parser = argparse.ArgumentParser() | ||
# parser.add_argument( | ||
# "--bmat_folder", | ||
# type=str, | ||
# required=True, | ||
# help=""" | ||
# All the bmat file should be formated as: | ||
# TargetSeq-TREATMENT_x_REP_x_REGIONID_x_CUTOFF_x.bmat.gz | ||
# and put in this folder. | ||
# """, | ||
# ) | ||
# parser.add_argument( | ||
# "--out", | ||
# type=str, | ||
# default=None, | ||
# help=""" | ||
# The path to put out csv file. | ||
# """, | ||
# ) | ||
# return parser.parse_args() | ||
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# def return_base_ratio(x): | ||
# if x["ref_base"] == "C": | ||
# return x["T"] / x["depth"] | ||
# elif x["ref_base"] == "G": | ||
# return x["A"] / x["depth"] | ||
# else: | ||
# return np.NaN | ||
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# if __name__ == "__main__": | ||
# # logging_setting | ||
# logging_setting() | ||
# # argparse_setting | ||
# args = argparse_setting() | ||
# pt_bmat = args.bmat_folder | ||
# pt_out = args.out | ||
# ls_bmat = [os.path.join(pt_bmat, i) for i in os.listdir(pt_bmat) if "bmat" in i] | ||
# # # TargetSeq-TREATMENT_x_REP_x_REGIONID_x_CUTOFF_x.bmat.gz | ||
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# # """ load bmat and merge """ | ||
# df_merge = pd.DataFrame() | ||
# for path in ls_bmat: | ||
# df = pd.read_csv(path, header=0, index_col=False, sep="\t", dtype=dt_type) | ||
# df["bmat_name"] = path.replace("bmat/", "") | ||
# df_merge = pd.concat([df_merge, df], ignore_index=True) | ||
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# # fix region_id for ND4 ND5.1 ND6 | ||
# # df_merge.chr_name = df_merge.chr_name.map(lambda x: x.replace("SHARE", "share")) | ||
# # df_merge.chr_name = df_merge.chr_name.map(lambda x: x.replace("ONLY", "only")) | ||
# # """ fix rep info """ | ||
# # remove no need cols | ||
# df = df_merge[ | ||
# [ | ||
# "chr_name", | ||
# "chr_index", | ||
# "ref_base", | ||
# "A", | ||
# "G", | ||
# "C", | ||
# "T", | ||
# "mut_num", | ||
# "bmat_name", | ||
# ] | ||
# ].copy() | ||
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# # form rep info | ||
# df["rep"] = df.bmat_name.map( | ||
# lambda x: "rep" + x.split("_REP_")[1].split("_CUTOFF_")[0] | ||
# ) | ||
# # form treatment info | ||
# df["treatment"] = df.bmat_name.map( | ||
# lambda x: x.split("-TREATMENT_")[1].split("_REP_")[0] | ||
# ) | ||
# # form cutoff info | ||
# df["cutoff"] = df.bmat_name.map( | ||
# lambda x: int(x.split("_CUTOFF_")[1].split("_REGIONID_")[0]) | ||
# ) | ||
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# if df.isna().sum().sum() != 0: | ||
# raise ValueError("bmat file name should be formated") | ||
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# # select | ||
# df_select = df[ | ||
# [ | ||
# "chr_name", | ||
# "chr_index", | ||
# "ref_base", | ||
# "A", | ||
# "G", | ||
# "C", | ||
# "T", | ||
# "bmat_name", | ||
# "rep", | ||
# "treatment", | ||
# "cutoff", | ||
# ] | ||
# ].copy() | ||
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# # df_select | ||
# df_select["depth"] = df_select[["A", "G", "C", "T"]].apply(sum, axis=1) | ||
# # 防止分母为0 | ||
# df_select.depth = df_select.depth.map(lambda x: x if x != 0 else 1) | ||
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# df_bmat_melt = df_select.melt( | ||
# id_vars=[ | ||
# "treatment", | ||
# "rep", | ||
# "cutoff", | ||
# "chr_name", | ||
# "ref_base", | ||
# "chr_index", | ||
# "bmat_name", | ||
# "depth", | ||
# ], | ||
# value_vars=["A", "G", "C", "T"], | ||
# var_name="mut_base", | ||
# value_name="mut_count", | ||
# ) | ||
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# df_bmat_melt.columns = [ | ||
# "treatment", | ||
# "rep", | ||
# "cutoff", | ||
# "region_id", | ||
# "ref_base", | ||
# "relative_pos", | ||
# "bmat_name", | ||
# "total_count", | ||
# "mut_base", | ||
# "mut_count", | ||
# ] | ||
# df_bmat_melt.to_csv(pt_out, index=False, header=True) | ||
# logging.info("script done.") |
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