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skjandu authored Feb 8, 2024
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## Introduction

differentialBindingAnalysis plots the enrichment scores (e-scores) from two PBM datasets against each other. This allows us to assess whether differential binding occurs between the two transcription factors.
`differentialBindingAnalysis` plots the enrichment scores (e-scores) from two PBM datasets against each other. This allows us to assess whether differential binding occurs between the two transcription factors.


## Methodology

The raw PBM datasets for two transcription factors are downloaded from uniPROBE. For each file, the user indicates the columns of the forward k-mer sequences and e-scores. For each k-mer sequence, its e-score from the first PBM file is plotted against its e-score from the second PBM file. Therefore, each data point in the plot is a k-mer with the ordered pair: (PBM 1 e-score, PBM 2 e-score). To indicate whether differential binding occurs, the resulting scatterplot can have either a trendline of the data points or a line with a slope of 1.
The raw PBM datasets for two transcription factors are downloaded from [uniPROBE](http://the_brain.bwh.harvard.edu/uniprobe/). For each file, the user indicates the columns of the forward k-mer sequences and e-scores. For each k-mer sequence, its e-score from the first PBM file is plotted against its e-score from the second PBM file. Therefore, each data point in the plot is a k-mer with the ordered pair: (PBM 1 e-score, PBM 2 e-score). To indicate whether differential binding occurs, the resulting scatterplot can have either a trendline of the data points or a line with a slope of 1.

## Parameters

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