U19 Python Pipeline

The U19-pipeline_python repository defines the DataJoint tables for the U19 projects. There is a companion MATLAB DataJoint pipeline where much of this repository has mirrored table definitions.

Installation

• The following instructions will detail two types of installation methods.
  1. User installation to access and fetch data from the database.
  2. Developer installation to set up the pipeline for running analysis and fetching data.

Recommended prerequisites

• The following prerequisites are recommended for both installation methods.

  Click to expand details

  Install an integrated development environment

  • DataJoint development and use can be done with a plain text editor in the terminal. However, an integrated development environment (IDE) can improve your experience. Several IDEs are available.

  • In this setup example, we will use Microsoft's Visual Studio Code. Installation instructions here.

  • Install the Jupyter extension for VS Code.

  Connect to PNI resources

  • The Princeton Neuroscience Institute (PNI) provides computing resources. You can optionally use these resources or setup the pipeline on your local machine.

  • Spock is the high performance computational cluster

    ssh -XY [email protected]
    

  • Scotty is used for interactive sessions

    ssh -XY [email protected]
    

  Install a virtual environment

  • A virtual environment allows you to install the packages required for a specific project within an isolated environment on your computer.

  • It is highly recommended to create a virtual environment to run the workflow.

  • Conda and virtualenv are virtual environment managers and you can use either option. Below are the commands for Conda.

  • If you are setting up the pipeline on your local machine follow the instructions below for Conda. If you are using spock.pni.princeton.edu or scotty.pni.princeton.edu, Conda is preinstalled and you can access it by running module load anacondapy/2021.11.

  • We will install Miniconda which is a minimal installer for conda.

    • Select the Miniconda installer link for your operating system and follow the instructions.

    • You may need to add the Miniconda directory to the PATH environment variable

      • First locate the Miniconda directory

      • Then modify and run the following command

        export PATH="<absolute-path-to-miniconda-directory>/bin:$PATH"

  • Create a new conda environment

    • Type the following command into a terminal window

      conda create -n <environment_name> python=<version>

    • Example command to create a conda environment

      conda create -n U19-pipeline_python python=3.8.11

  • Activate the conda environment

    conda activate <environment_name>

  Install git

  • Linux and Mac operating systems come preinstalled with Git. If running in Windows get Git.

  Install graphviz

  • To display DataJoint Diagrams, install graphviz.

User installation

• The following instructions will allow a user to access and fetch data from the database.

  Click to expand details

  Install DataJoint

  • Activate the conda environment

    conda activate <environment_name>

  • Install DataJoint

  pip install datajoint

  Access the database

  • In a new Jupyter notebook, run the following commands.

    import getpass
    import datajoint as dj
    
    dj.config['database.host'] = 'datajoint00.pni.princeton.edu'
    dj.config['database.user'] = '<username>'
    dj.config['database.password'] = getpass.getpass() # enter the password securily
    
    scan = dj.create_virtual_module('scan', 'u19_scan_element')
    imaging = dj.create_virtual_module('imaging', 'u19_imaging_element')
    
    probe = dj.create_virtual_module('probe', 'u19_probe_element')
    ephys = dj.create_virtual_module('ephys', 'u19_ephys_element')
    

  • Now that the virtual modules are created to access the tables in the database, you can query and fetch from the database.

Developer installation

• The following instructions will allow a user to set up the pipeline for running analysis and fetching data.

  Click to expand details

  Fork and clone the repository

  • In a broswer, navigate to the BrainCOGS/U19-pipeline_python repository and fork this repository.

  • In a terminal window, clone your fork of the repository to your local machine.

    git clone https://github.com/<GitHub username>/U19-pipeline_python.git
    

  • If you cannot clone repositories with ssh, set keys.

  Install the repository

  • Activate the conda environment

  conda activate <environment_name>

  • Change directory to this repository

  cd U19-pipeline_python

  • Install this repository in editable mode

  pip install -e .

  Configure the DataJoint connection to the database

  • See the following Jupyter notebook to configure DataJoint. notebooks/00-datajoint-configuration.ipynb

  • Ephys element and imaging element require root paths for ephys and imaging data. Here are the notebooks showing how to set up the configurations properly.

    • Ephys element configuration
    • Imaging element configuration

Tutorials

We have created some tutorial notebooks to help you start working with DataJoint.

1. Querying data

   • jupyter notebook notebooks/tutorials/1-Explore U19 data pipeline with DataJoint.ipynb

2. Building analysis pipelines

   • Recommended if you are going to create new databases or tables for analysis.
   • jupyter notebook notebooks/tutorials/2-Analyze data with U19 pipeline and save results.ipynb
   • jupyter notebook notebooks/tutorials/3-Build a simple data pipeline.ipynb

Accessing data files on your system

• There are several data files (behavior, imaging & electrophysiology) that are referenced in the database.

• To access thse files you should mount PNI file server volumes on your system.

• There are three main file servers across PNI where data is stored (braininit, Bezos & u19_dj).

  Click to expand details

  On windows systems

  • From Windows Explorer, select "Map Network Drive" and enter:
    \\cup.pni.princeton.edu\braininit\ (for braininit)
    \\cup.pni.princeton.edu\Bezos-center\ (for Bezos)
    \\cup.pni.princeton.edu\u19_dj\ (for u19_dj)
    
  • Authenticate with your NetID and PU password (NOT your PNI password, which may be different). When prompted for your username, enter PRINCETON\netid (note that PRINCETON can be upper or lower case) where netid is your PU NetID.

  On OS X systems

  • Select "Go->Connect to Server..." from Finder and enter:
    smb://cup.pni.princeton.edu/braininit/ (for braininit)
    smb://cup.pni.princeton.edu/Bezos-center/ (for Bezos)
    smb://cup.pni.princeton.edu/u19_dj/ (for u19_dj)
    
  • Authenticate with your NetID and PU password (NOT your PNI password, which may be different).

  On Linux systems

  • Follow extra steps depicted in this link: https://npcdocs.princeton.edu/index.php/Mounting_the_PNI_file_server_on_your_desktop

  Notable data

  Here are some shortcuts to common used data accross PNI

  Sue Ann's Towers Task

  • Imaging: /Bezos-center/RigData/scope/bay3/sakoay/{protocol_name}/imaging/{subject_nickname}/
  • Behavior: /braininit/RigData/scope/bay3/sakoay/{protocol_name}/data/{subject_nickname}/

  Lucas Pinto's Widefield

  • Imaging /braininit/RigData/VRwidefield/widefield/{subject_nickname}/{session_date}/
  • Behavior /braininit/RigData/VRwidefield/behavior/lucas/blocksReboot/data/{subject_nickname}/

  Lucas Pinto's Opto inactivacion experiments

  • Imaging /braininit/RigData/VRLaser/LaserGalvo1/{subject_nickname}/
  • Behavior /braininit/RigData/VRLaser/behav/lucas/blocksReboot/data/{subject_nickname}/

  Get path info for the session behavioral file

  1. Mount needed file server
  2. Connect to the Database
  3. Create a structure with subject_fullname and session_date from the session
     key['subject_fullname'] = 'koay_K65'
key['session_Date'] = '2018-02-05'
     
  4. Fetch filepath info: data_dir = (acquisition.SessionStarted & key).fetch('remote_path_behavior_file')
     

Major schemas in the pipeline

Click to expand details

lab

reference

subject

action

acquisition

task

behavior

Behavior data for Towers task.

ephys_element

• Ephys related tables were created with DataJoint Element Array Ephys, processing ephys data aquired with SpikeGLX and pre-processed by Kilosort2. For this pipeline we are using the (acute) ephys module from element-array-ephys.

imaging

• Imaging pipeline processed with customized algorithm for motion correction and CNMF for cell segmentation in matlab.

scan_element and imaging_element

• Scan and imaging tables created with DataJoint Element Calcium Imaging, processing imaging data acquired with ScanImage and pre-processed by Suite2p.

Datajoint features

Import datajoint as follows:

import datajoint as dj

Update a table entry

dj.Table._update(schema.Table & key, 'column_name', 'new_data')

Get list of all column names in a table (without having to issue a query or fetch)

table.heading.attributes.keys()

This also works on a query object:

schema = dj.create_virtual_module("some_schema","some_schema")
query_object = schema.Sample() & 'sample_name ="test"'
query_object.heading.attributes.keys()