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Merging development branch from Malaria project - decontamination, pipelines for organisms with no variant truth set, etc. #451

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@brosula brosula commented Apr 24, 2024

Merging work based on the development I have been working on for the short read malaria project. Most updates are in line with jts_updates_for_malaria_joint_calling branch as of 4/23/2024.

Major updates:

  1. Addition of "bootstrap" WDLs to support the processing of organisms without a variant truth set.
  • SRWholeGenomeBootstrap.wdl - Disabled VETS
  • SRJointCallGVCFsWithGenomicsDBBootstrap.wdl - Disabled VETS
  1. Addition of bowtie2-based decontamination
  • SRFlowcellDecontaminate.wdl - Add "aligner" as a parameter that can take "bwa-mem2" or "bowtie2"
  • SRUtils.wdl - Added bowtie2 task for alignment

Minor updates:

  1. FunctionalAnnotation.wdl - Added support for non-Plasmodium reference genomes
  2. VariantUtils.wdl - Added various tasks, such as hard filtering by GATK defaults
  3. HaplotypeCallerBootstrap.wdl - Added to support SRWholeGenomeBootstrap.wdl (although it is largely similar to HaplotypeCaller.wdl)
  4. SRReblockGVCFs.wdl - Added standalone reblocking workflow to process (g)vcfs produced by HaplotypeCaller when ReblockGVCFs was disabled.

@brosula brosula requested a review from jonn-smith April 24, 2024 16:31
brosula and others added 29 commits April 26, 2024 11:55
…DBBootstrap

( no sharding of snpeff_db implemented )
Report the correct coverage metric when given a BED file.
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3 participants