Help with rmats2sashimiplot

[Sahu-Mousumi]

Hi!

Below is my rmats command

rmats.py --b1 b1.txt --b2 b2.txt --gtf gencode.v44.annotation.gtf --novelSS --allow-clipping -t paired --libType fr-firststrand --readLength 76 --variable-read-length --nthread $SLURM_CPUS_PER_TASK --od rmats_res --tmp tmp_rmats_res

I found CD58 is statistically significant-

chr chr1
strand -
exonStart_0base 116536096
exonEnd 116536228
upstreamES 116521905
upstreamEE 116521983
downstreamES 116544310
downstreamEE 116544604
ID.1 151386
IJC_SAMPLE_1 45,49,64,35
SJC_SAMPLE_1 0,2,0,0
IJC_SAMPLE_2 38,49,65,53,38,38
SJC_SAMPLE_2 1,6,0,3,0,5
IncFormLen 150
SkipFormLen 75
PValue 0.00028013
FDR 0.04768118

But while plotting shahimi using rMATS2Shashimiplot, I am generating below error-

File "/home/.local/lib/python3.10/site-packages/MISO/misopy/sashimi_plot/sashimi_plot.py", line 140

raise Exception, "Cannot find file %s. Are you sure the events " \

Should I use any filter or any suggestion??

Thanks
Mousumi

[EricKutschera]

There are a couple other posts that had the Cannot find file error:
#61
#62

In both of those cases there was an earlier error message that was the main issue. The particular issue in those other posts is fixed in v3.0.0

Can you post the full output from rmats2sashimiplot?