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dev updates Tue 8 Sep 2015 13:33:32 BST
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Expand Up @@ -18,7 +18,7 @@ NGSeasy (beta): A [Dockerized](https://www.docker.com/) NGS pipeline and tool-bo

[**NGSeasy is completely open source and we encourage interested folks to jump in and get involved in the dev with us.**](https://github.com/KHP-Informatics/ngseasy.git)

## NGSeasy: Genome Comparison & Analytic Testing ([GCAT](http://www.bioplanet.com/gcat)) Reports
## NGSeasy: Genome Comparison & Analytic Testing ([GCAT](http://www.bioplanet.com/gcat)) Reports
Here we provide a quick look at basic NGSeasy performance (more results coming soon).

|GCAT Report|Test Data |Pipeline|
Expand Down Expand Up @@ -48,7 +48,7 @@ Please contact us for help/guidance on using the beta release.

**Please Direct all queries to [https://github.com/KHP-Informatics/ngseasy/issues]**

When sending bug reports etc please provide:-
When sending bug reports etc please provide:-

- Date of Download
- OS and version
Expand Down Expand Up @@ -148,7 +148,7 @@ git clone https://github.com/KHP-Informatics/ngseasy.git

- Default install directory is `/home/${USER}`
- in this example user home is `/home/ec2-user`
- `make INTSALLDIR="/home/ec2-user" all`
- `make INTSALLDIR="/home/ec2-user" all`
- sets up top level directory structure
- gets all docker images
- gets indexed hg19 and b37 genomes
Expand All @@ -167,7 +167,7 @@ cd ngseasy
## 1.
make INTSALLDIR="/home/ec2-user" all

## 2.
## 2.
sudo make install
```

Expand Down Expand Up @@ -204,15 +204,15 @@ Upload: 5.18 Mbit/s

## Install time on Amazon EC2

Connection Speed: ~ 800 Mbit/s
Connection Speed: ~ 800 Mbit/s

```
real 94m54.237s
user 12m26.960s
sys 28m46.648s
```

Note: We have only tested NGSeasy installation on Amazon EC2, Openstack and UK University Networks. These are all fairly fast networks with speeds exceeding 800 Mbit/s on average.
Note: We have only tested NGSeasy installation on Amazon EC2, Openstack and UK University Networks. These are all fairly fast networks with speeds exceeding 800 Mbit/s on average.

## Running NGSeasy for the first time on the test data

Expand Down Expand Up @@ -275,6 +275,14 @@ We have adapted the current best practices from the Genome Analysis Toolkit (GAT

As the containers themselves can be run as executables with pre-specified cpu and RAM resources, the orchestration of the pipeline can be placed under the control of conventional load balancers if this mode is required.

## Genomes

| Genome |
|--------|
|hs37d5|
|b37|
|hg19|
|hs38DH|

****************

Expand Down Expand Up @@ -577,7 +585,7 @@ ngs_projects
|__raw_fastq
|__config_files
|__run_logs
|__ngseasy_resources
|__ngseasy_resources
|
|__ project_id
|
Expand Down Expand Up @@ -777,4 +785,4 @@ sudo docker build -t compbio/ngseasy-snpeff:${VERSION} .
- http://plagnol-lab.blogspot.co.uk/2013/11/faq-and-clarifications-for-exomedepth-r.html

*************************
(C) 2015 Stephen J Newhouse & Amos Folarin
(C) 2015 Stephen J Newhouse & Amos Folarin

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