A plugin for ImageJ, the standard in scientific image processing, that uses automated Sholl to perform neuronal morphometry directly from bitmap images.
Sholl analysis (Sholl, D.A., 1953) is a method used by neuroanatomists to describe neuronal arbors. The plugin takes the original technique beyond conventional approaches, offering major advantages over other implementations:
- It does not require previous tracing of the arbor (although analysis can be applied to previously traced arbors)
- It combines curve fitting with several methods to automatically retrieve quantitative descriptors from sampled data
- It allows continuous and repeated sampling around user-defined foci
Why ASA? Throughout 2012 the plugin was [temporarily](SHA: 1fdf4992b748ef8678f57601f2739473e40718c9) called Advanced Sholl Analysis, hence the acronym