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Merge branch 'u/morriscb/addAtlasIds' into rc/0.3.0
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morriscb committed Oct 31, 2024
2 parents 8b52e7c + 8e56c9e commit 4626887
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2 changes: 1 addition & 1 deletion .github/workflows/abc_atlas_access_ci.yaml
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Expand Up @@ -11,7 +11,7 @@ jobs:
strategy:
matrix:
os: [ "macos-latest", "windows-latest", "ubuntu-latest" ]
python-version: [ "3.8", "3.9", "3.10", "3.11" ]
python-version: [ "3.8", "3.9", "3.10", "3.11", "3.12" ]
fail-fast: false
steps:
- uses: actions/checkout@v4
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2 changes: 1 addition & 1 deletion _toc.yml
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Expand Up @@ -8,7 +8,7 @@ parts:
chapters:
- file: notebooks/getting_started
- file: notebooks/general_accessing_10x_snRNASeq_tutorial

- file: notebooks/abc_atlas_selection_example
- caption: DATA
chapters:
- file: descriptions/WMB_dataset
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6 changes: 3 additions & 3 deletions descriptions/MERFISH-C57BL6J-638850.md
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Expand Up @@ -9,10 +9,10 @@ resolution transcriptomic and spatial atlas of cell types described in
The expression matrices and associated metadata is hosted on AWS S3 bucket as a
AWS Public Dataset:

| Component | Current Version | Size |
|---|--|---|
| Component | Current Version | Size |
|---|--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|---|
| Expression Matrices | [s3://allen-brain-cell-atlas/expression_matrices/MERFISH-C57BL6J-638850/20230830](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#expression_matrices/MERFISH-C57BL6J-638850/20230830/) | 14.2 GB |
| Metadata | [s3://allen-brain-cell-atlas/metadata/MERFISH-C57BL6J-638850/20231215](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/MERFISH-C57BL6J-638850/20231215/) | 1.81 GB |
| Metadata | [s3://allen-brain-cell-atlas/metadata/MERFISH-C57BL6J-638850/20241115](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/MERFISH-C57BL6J-638850/20241115/) | 1.81 GB |

Data is being share under the CC BY NC 4.0 license.

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2 changes: 1 addition & 1 deletion descriptions/WHB-10X-clustering.md
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Expand Up @@ -11,7 +11,7 @@ The associated metadata is hosted on AWS S3 bucket as a AWS Public Dataset:

| Component | Current Version | Size |
|------------------------|--------------------------------------------------------------------------------------------------------------------------------------------------------------|----------|
| Cell and gene metadata | [s3://allen-brain-cell-atlas/metadata/WHB-10Xv3/20240330](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/WHB-10Xv3/20240330/) | 862.1 MB |
| Cell and gene metadata | [s3://allen-brain-cell-atlas/metadata/WHB-10Xv3/20241115](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/WHB-10Xv3/20241115/) | 862.1 MB |

Data is being share under the CC BY NC 4.0 license.

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2 changes: 2 additions & 0 deletions descriptions/WHB-10Xv3.md
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Expand Up @@ -12,6 +12,8 @@ a AWS Public Dataset:
| Component | Current Version | Size |
|---|--|---------|
| Expression Matrices | [s3://allen-brain-cell-atlas/expression_matrices/WHB-10Xv3/20240330/](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#expression_matrices/WHB-10Xv3/20240330/) | 70.0 GB |
| Cell and gene metadata | [s3://allen-brain-cell-atlas/metadata/WHB-10Xv3/20241115](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/WHB-10Xv3/20241115/) | 862.1 MB |


Data is being share under the CC BY NC 4.0 license.

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2 changes: 1 addition & 1 deletion descriptions/WMB-10X.md
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Expand Up @@ -11,7 +11,7 @@ The associated metadata is hosted on AWS S3 bucket as a AWS Public Dataset:

| Component | Current Version | Size |
|---|--|--|
| Metadata | [s3://allen-brain-cell-atlas/metadata/WMB-10X/20231215](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/WMB-10X/20231215/) | 2.39 GB |
| Metadata | [s3://allen-brain-cell-atlas/metadata/WMB-10X/20241115](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/WMB-10X/20241115/) | 2.39 GB |

Data is being share under the CC BY NC 4.0 license.

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6 changes: 3 additions & 3 deletions descriptions/WMB-10XMulti.md
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Expand Up @@ -8,9 +8,9 @@ a high resolution transcriptomic and spatial atlas of cell types described in
The expression matrices and associated metadata is hosted on AWS S3 bucket as a
AWS Public Dataset:

| Component | Current Version | Size |
|---|--|--|
| Expression Matrices | [s3://allen-brain-cell-atlas/expression_matrices/WMB-10XMulti/20230830](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#expression_matrices/WMB-10Xv3/20230630/) | 176.31 GB |
| Component | Current Version | Size |
|---|------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|--------|
| Expression Matrices | [s3://allen-brain-cell-atlas/expression_matrices/WMB-10XMulti/20230830](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#expression_matrices/WMB-10XMulti/20230830/) | 211 MB |

Data is being share under the CC BY NC 4.0 license.

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8 changes: 4 additions & 4 deletions descriptions/Zhuang-ABCA-1.md
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Expand Up @@ -39,11 +39,11 @@ downloaded from the CCF coordinate file in the AWB bucket. Refer to
The expression matrices and associated metadata is hosted on AWS S3 bucket as a
AWS Public Dataset:

| Component | Current Version | Size |
|---|--|---|
| Component | Current Version | Size |
|---|---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|---|
| Expression Matrices | [s3://allen-brain-cell-atlas/expression_matrices/Zhuang-ABCA-1/20230830/](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#expression_matrices/Zhuang-ABCA-1/20230830/) | 3.09 GB |
| Metadata | [s3://allen-brain-cell-atlas/metadata/Zhuang-ABCA-1/20231215](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/Zhuang-ABCA-1/20231215/) | 1.33 GB |
| CCF Coordinates | [s3://allen-brain-cell-atlas/metadata/Zhuang-ABCA-1-CCF/20230830](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/Zhuang-ABCA-1-CCF/20230830/) | 0.21 GB |
| Metadata | [s3://allen-brain-cell-atlas/metadata/Zhuang-ABCA-1/20241115](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/Zhuang-ABCA-1/20241115/) | 1.33 GB |
| CCF Coordinates | [s3://allen-brain-cell-atlas/metadata/Zhuang-ABCA-1-CCF/20230830](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/Zhuang-ABCA-1-CCF/20230830/) | 0.21 GB |

Data is being share under the CC BY 4.0 license.

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2 changes: 1 addition & 1 deletion descriptions/Zhuang-ABCA-2.md
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Expand Up @@ -41,7 +41,7 @@ AWS Public Dataset:
| Component | Current Version | Size |
|---|--|---|
| Expression Matrices | [s3://allen-brain-cell-atlas/expression_matrices/Zhuang-ABCA-2/20230830/](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#expression_matrices/Zhuang-ABCA-2/20230830/) | 1.30 GB |
| Metadata | [s3://allen-brain-cell-atlas/metadata/Zhuang-ABCA-2/20231215](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/Zhuang-ABCA-2/20231215/) | 0.57 GB |
| Metadata | [s3://allen-brain-cell-atlas/metadata/Zhuang-ABCA-2/20241115](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/Zhuang-ABCA-2/20241115/) | 0.57 GB |
| CCF Coordinates | [s3://allen-brain-cell-atlas/metadata/Zhuang-ABCA-2-CCF/20230830](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/Zhuang-ABCA-2-CCF/20230830/) | 0.08 GB |

Data is being share under the CC BY 4.0 license.
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2 changes: 1 addition & 1 deletion descriptions/Zhuang-ABCA-3.md
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Expand Up @@ -38,7 +38,7 @@ AWS Public Dataset:
| Component | Current Version | Size |
|---|--|---|
| Expression Matrices | [s3://allen-brain-cell-atlas/expression_matrices/Zhuang-ABCA-3/20230830/](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#expression_matrices/Zhuang-ABCA-3/20230830/) | 1.69 GB |
| Metadata | [s3://allen-brain-cell-atlas/metadata/Zhuang-ABCA-3/20231215](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/Zhuang-ABCA-3/20231215/) | 0.75 GB |
| Metadata | [s3://allen-brain-cell-atlas/metadata/Zhuang-ABCA-3/20241115](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/Zhuang-ABCA-3/20241115/) | 0.75 GB |
| CCF Coordinates | [s3://allen-brain-cell-atlas/metadata/Zhuang-ABCA-3-CCF/20230830](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/Zhuang-ABCA-3-CCF/20230830/) | 0.12 GB |

Data is being share under the CC BY 4.0 license.
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2 changes: 1 addition & 1 deletion descriptions/Zhuang-ABCA-4.md
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Expand Up @@ -33,7 +33,7 @@ AWS Public Dataset:
| Component | Current Version | Size |
|---|--|---|
| Expression Matrices | [s3://allen-brain-cell-atlas/expression_matrices/Zhuang-ABCA-4/20230830/](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#expression_matrices/Zhuang-ABCA-4/20230830/) | 0.16 GB |
| Metadata | [s3://allen-brain-cell-atlas/metadata/Zhuang-ABCA-4/20231215](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/Zhuang-ABCA-4/20231215/) | 0.08 GB |
| Metadata | [s3://allen-brain-cell-atlas/metadata/Zhuang-ABCA-4/20241115](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/Zhuang-ABCA-4/20241115/) | 0.08 GB |
| CCF Coordinates | [s3://allen-brain-cell-atlas/metadata/Zhuang-ABCA-4-CCF/20230830](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/Zhuang-ABCA-4-CCF/20230830/) | 0.01 GB |

Data is being share under the CC BY 4.0 license.
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28 changes: 22 additions & 6 deletions intro.md
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Expand Up @@ -11,14 +11,14 @@ understanding of this amazing organ in ways we never thought possible. The
ABC Atlas can be accessed at [here](https://portal.brain-map.org/atlases-and-data/bkp/abc-atlas)
and is the primary method of interacting with these data. Any questions or
issues associated with the ABC Atlas are best directed to the [Allen Institute
Commnituy Forum](https://community.brain-map.org/). This repository is intended
Community Forum](https://community.brain-map.org/). This repository is intended
for users who wish to download the ABC Atlas data and processes it locally.

Data associated with the ABC Atlas is hosted on Amazon Web Services (AWS) in an
S3 bucket as a AWS Public Dataset, [arn:aws:s3:::allen-brain-cell-atlas](https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html).
No account or login is required for access. The purpose of this repo is to
No account or login is required for access. ***The purpose of this repo is to
provide an overview of the available data, how to download and use it through
example use cases.
example use cases.***

The spring 2024 public beta data release includes:
* **[Mouse whole-brain transcriptomic cell type atlas](descriptions/WMB_dataset.md)
Expand All @@ -41,11 +41,18 @@ subdivided into smaller packages grouped by method and anatomical origin. The
notebooks provide example code on how to access data across these individual
files using the AbcProjectCache.

Available notebooks:
If you have used the ABC Atlas visualization to select cells from a dataset and
with to use your selected cells in an analysis with the data available for
download here, you find a tutorial to do so in the
[Using cells selected in the ABC Atlas](notebooks/abc_atlas_selection_example.ipynb)
notebook.

## Available notebooks:

* [**Getting started**](notebooks/getting_started.ipynb): learn how to use the
AbcProjectCache to facilitate data download and usage.
[*notebooks/getting_started.ipynb*]
* [**Using cells selected in the ABC Atlas**](notebooks/abc_atlas_selection_example.ipynb): learn how
to use cells selected from an ABC Atlas visualization in your analysis.
* [**Loading genes from expression matrix data**](notebooks/general_accessing_10x_snRNASeq_tutorial.ipynb):
learn how to select genes from 10X expression matricies and combine them with
the cell metadata.
Expand All @@ -58,10 +65,19 @@ Available notebooks:
(Kimberly Siletti)**

## Release Notes
* **[ABC Sample ID update (version 20241115) ab_atlas_access (v0.3.0)]**
* Add abc_sample_id to cell_metadatas of the projects listed below. This
allows users to use cell selections they've downloaded from the ABC Atlas
visualization.
* MERFISH-C57BL6J-638850(-imputed)
* WHB-10Xv3
* WMB-10X
* Zhuang-ABCA-(1-4)
* Added notebook showing how to use abc_sample_id.
* **[Summer 2024 Public Beta (version 20240831) ab_atlas_access (v0.2.0)]**
* Released ~8k imputed genes for the MERFISH-C57BL6J-638850 dataset.
* Added new notebooks and pages for the imputed gene dataset.
* Updated cache object for better compatiblity across platforms.
* Updated cache object for better compatibility across platforms.
* Added automatic unittesting via GitHub Actions.
* Modified cache to autodetect cache type (local or s3) for easier
compatibility with CodeOcean/s3fs-fuse mounts.
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