diff --git a/.github/workflows/bsim-tests.yaml b/.github/workflows/bsim-tests.yaml
index a8a49be767080b3..f52a0439d20ba34 100644
--- a/.github/workflows/bsim-tests.yaml
+++ b/.github/workflows/bsim-tests.yaml
@@ -42,6 +42,7 @@ jobs:
       EDTT_PATH: ../tools/edtt
       bsim_bt_52_test_results_file: ./bsim_bt/52_bsim_results.xml
       bsim_bt_53_test_results_file: ./bsim_bt/53_bsim_results.xml
+      bsim_bt_53split_test_results_file: ./bsim_bt/53_bsim_split_results.xml
       bsim_net_52_test_results_file: ./bsim_net/52_bsim_results.xml
     steps:
       - name: Apply container owner mismatch workaround
@@ -134,15 +135,25 @@ jobs:
         if: steps.check-bluetooth-files.outputs.any_changed == 'true' || steps.check-common-files.outputs.any_changed == 'true'
         run: |
           export ZEPHYR_BASE=${PWD}
-          WORK_DIR=${ZEPHYR_BASE}/bsim_bt nice tests/bsim/bluetooth/compile.sh
+          export WORK_DIR=${ZEPHYR_BASE}/bsim_bt
+          # Build and run the BT tests for nrf52_bsim:
+          nice tests/bsim/bluetooth/compile.sh
           RESULTS_FILE=${ZEPHYR_BASE}/${bsim_bt_52_test_results_file} \
-          SEARCH_PATH=tests/bsim/bluetooth/ tests/bsim/run_parallel.sh
-          # Run the BT controller tests also for the nrf5340
+          TESTS_FILE=tests/bsim/bluetooth/tests.nrf52bsim.txt tests/bsim/run_parallel.sh
+          # Build and run the BT controller tests also for the nrf5340bsim_nrf5340_cpunet
           BOARD=nrf5340bsim_nrf5340_cpunet \
-          WORK_DIR=${ZEPHYR_BASE}/bsim_bt nice tests/bsim/bluetooth/ll/compile.sh
+          nice tests/bsim/bluetooth/compile.nrf5340bsim_nrf5340_cpunet.sh
           BOARD=nrf5340bsim_nrf5340_cpunet \
           RESULTS_FILE=${ZEPHYR_BASE}/${bsim_bt_53_test_results_file} \
-          SEARCH_PATH=tests/bsim/bluetooth/ll/ tests/bsim/run_parallel.sh
+          TESTS_FILE=tests/bsim/bluetooth/tests.nrf5340bsim_nrf5340_cpunet.txt \
+          tests/bsim/run_parallel.sh
+          # Build and run the nrf5340 split stack tests set
+          BOARD=nrf5340bsim_nrf5340_cpuapp \
+          nice tests/bsim/bluetooth/compile.nrf5340bsim_nrf5340_cpuapp.sh
+          BOARD=nrf5340bsim_nrf5340_cpuapp \
+          RESULTS_FILE=${ZEPHYR_BASE}/${bsim_bt_53split_test_results_file} \
+          TESTS_FILE=tests/bsim/bluetooth/tests.nrf5340bsim_nrf5340_cpuapp.txt \
+          tests/bsim/run_parallel.sh
 
       - name: Run Networking Tests with BSIM
         if: steps.check-networking-files.outputs.any_changed == 'true' || steps.check-common-files.outputs.any_changed == 'true'
@@ -160,6 +171,7 @@ jobs:
           path: |
             ./bsim_bt/52_bsim_results.xml
             ./bsim_bt/53_bsim_results.xml
+            ./bsim_bt/53_bsim_split_results.xml
             ./bsim_net/52_bsim_results.xml
             ${{ github.event_path }}
           if-no-files-found: warn
diff --git a/tests/bsim/bluetooth/compile.nrf5340bsim_nrf5340_cpuapp.sh b/tests/bsim/bluetooth/compile.nrf5340bsim_nrf5340_cpuapp.sh
new file mode 100755
index 000000000000000..76e912d87d7531f
--- /dev/null
+++ b/tests/bsim/bluetooth/compile.nrf5340bsim_nrf5340_cpuapp.sh
@@ -0,0 +1,24 @@
+#!/usr/bin/env bash
+# Copyright 2018 Oticon A/S
+# SPDX-License-Identifier: Apache-2.0
+
+# Compile all bluetooth applications needed for the split stack tests
+
+#set -x #uncomment this line for debugging
+set -ue
+: "${BSIM_COMPONENTS_PATH:?BSIM_COMPONENTS_PATH must be defined}"
+: "${ZEPHYR_BASE:?ZEPHYR_BASE must be set to point to the zephyr root\
+ directory}"
+
+WORK_DIR="${WORK_DIR:-${ZEPHYR_BASE}/bsim_out}"
+
+BOARD_ROOT="${BOARD_ROOT:-${ZEPHYR_BASE}}"
+BOARD="${BOARD:-nrf5340bsim_nrf5340_cpuapp}"
+
+mkdir -p ${WORK_DIR}
+
+source ${ZEPHYR_BASE}/tests/bsim/compile.source
+
+app=tests/bsim/bluetooth/ll/bis sysbuild=1 compile
+
+wait_for_background_jobs
diff --git a/tests/bsim/bluetooth/compile.nrf5340bsim_nrf5340_cpunet.sh b/tests/bsim/bluetooth/compile.nrf5340bsim_nrf5340_cpunet.sh
new file mode 100755
index 000000000000000..e8d78683b2e13e4
--- /dev/null
+++ b/tests/bsim/bluetooth/compile.nrf5340bsim_nrf5340_cpunet.sh
@@ -0,0 +1,23 @@
+#!/usr/bin/env bash
+# Copyright 2018 Oticon A/S
+# SPDX-License-Identifier: Apache-2.0
+
+# Compile all the applications needed by the Bluetooth bsim tests on the nrf5340_cpunet
+
+#set -x #uncomment this line for debugging
+set -ue
+: "${BSIM_COMPONENTS_PATH:?BSIM_COMPONENTS_PATH must be defined}"
+: "${ZEPHYR_BASE:?ZEPHYR_BASE must be set to point to the zephyr root\
+ directory}"
+
+WORK_DIR="${WORK_DIR:-${ZEPHYR_BASE}/bsim_out}"
+
+BOARD_ROOT="${BOARD_ROOT:-${ZEPHYR_BASE}}"
+
+mkdir -p ${WORK_DIR}
+
+source ${ZEPHYR_BASE}/tests/bsim/sh_common.source
+
+${ZEPHYR_BASE}/tests/bsim/bluetooth/ll/compile.sh
+
+wait_for_background_jobs
diff --git a/tests/bsim/bluetooth/tests.nrf52bsim.txt b/tests/bsim/bluetooth/tests.nrf52bsim.txt
new file mode 100644
index 000000000000000..9b76be4ec7500a5
--- /dev/null
+++ b/tests/bsim/bluetooth/tests.nrf52bsim.txt
@@ -0,0 +1,3 @@
+# Search paths(s) for tests which will be run in the nrf52bsim
+# This file is used in CI to select which tests are run
+tests/bsim/bluetooth/
diff --git a/tests/bsim/bluetooth/tests.nrf5340bsim_nrf5340_cpuapp.txt b/tests/bsim/bluetooth/tests.nrf5340bsim_nrf5340_cpuapp.txt
new file mode 100644
index 000000000000000..f8f65fbeb73b90e
--- /dev/null
+++ b/tests/bsim/bluetooth/tests.nrf5340bsim_nrf5340_cpuapp.txt
@@ -0,0 +1,3 @@
+# Search paths(s) for tests which will be run in the nrf5340 split stack configuration
+# This file is used in CI to select which tests are run
+tests/bsim/bluetooth/ll/bis/tests_scripts/broadcast_iso.sh
diff --git a/tests/bsim/bluetooth/tests.nrf5340bsim_nrf5340_cpunet.txt b/tests/bsim/bluetooth/tests.nrf5340bsim_nrf5340_cpunet.txt
new file mode 100644
index 000000000000000..1d9d73630db5c9c
--- /dev/null
+++ b/tests/bsim/bluetooth/tests.nrf5340bsim_nrf5340_cpunet.txt
@@ -0,0 +1,4 @@
+# Search paths(s) for tests which will be run in the nrf5340 net core (both app, host and controller
+# built in the net core)
+# This file is used in CI to select which tests are run
+tests/bsim/bluetooth/ll/