diff --git a/doc/develop/test/ztest.rst b/doc/develop/test/ztest.rst
index 1eff49906bf4f00..c7d3d23911e92c5 100644
--- a/doc/develop/test/ztest.rst
+++ b/doc/develop/test/ztest.rst
@@ -10,10 +10,6 @@ test structure.
 The framework can be used in two ways, either as a generic framework for
 integration testing, or for unit testing specific modules.
 
-To enable support for the latest Ztest API, set
-:kconfig:option:`CONFIG_ZTEST_NEW_API` to ``y``. There is also a legacy API
-that is deprecated and will eventually be removed.
-
 Creating a test suite
 *********************
 
@@ -581,7 +577,7 @@ Shuffling Test Sequence
 By default the tests are sorted and ran in alphanumerical order.  Test cases may
 be dependent on this sequence. Enable :kconfig:option:`CONFIG_ZTEST_SHUFFLE` to
 randomize the order. The output from the test will display the seed for failed
-tests.  For native posix builds you can provide the seed as an argument to
+tests.  For native simulator builds you can provide the seed as an argument to
 twister with `--seed`
 
 Static configuration of ZTEST_SHUFFLE contains:
@@ -592,7 +588,7 @@ Static configuration of ZTEST_SHUFFLE contains:
 
 Test Selection
 **************
-For POSIX enabled builds with ZTEST_NEW_API use command line arguments to list
+For tests built for native simulator, use command line arguments to list
 or select tests to run. The test argument expects a comma separated list
 of ``suite::test`` .  You can substitute the test name with an ``*`` to run all
 tests within a suite.
diff --git a/samples/sample_definition_and_criteria.rst b/samples/sample_definition_and_criteria.rst
index 5a75496aff60507..be0c9dfc3ef1f3d 100644
--- a/samples/sample_definition_and_criteria.rst
+++ b/samples/sample_definition_and_criteria.rst
@@ -19,7 +19,7 @@ Sample Criteria
   * The primary purpose of a sample is to provide a reference to the user.
   * Samples must not use Zephyr's testing framework.
 
-    * Must not use :kconfig:option:`CONFIG_ZTEST` or :kconfig:option:`CONFIG_ZTEST_NEW_API`.
+    * Must not use :kconfig:option:`CONFIG_ZTEST`
     * Must not use zasserts in samples.
 
   * If a sample can provide output that can be verified, then output should be evaluated against
diff --git a/scripts/tests/twister_blackbox/test_data/tests/dummy/agnostic/group1/subgroup1/prj.conf b/scripts/tests/twister_blackbox/test_data/tests/dummy/agnostic/group1/subgroup1/prj.conf
index 9228251051ec064..9467c2926896dd7 100644
--- a/scripts/tests/twister_blackbox/test_data/tests/dummy/agnostic/group1/subgroup1/prj.conf
+++ b/scripts/tests/twister_blackbox/test_data/tests/dummy/agnostic/group1/subgroup1/prj.conf
@@ -1,2 +1 @@
 CONFIG_ZTEST=y
-CONFIG_ZTEST_NEW_API=y
diff --git a/scripts/tests/twister_blackbox/test_data/tests/dummy/agnostic/group1/subgroup2/prj.conf b/scripts/tests/twister_blackbox/test_data/tests/dummy/agnostic/group1/subgroup2/prj.conf
index 9228251051ec064..9467c2926896dd7 100644
--- a/scripts/tests/twister_blackbox/test_data/tests/dummy/agnostic/group1/subgroup2/prj.conf
+++ b/scripts/tests/twister_blackbox/test_data/tests/dummy/agnostic/group1/subgroup2/prj.conf
@@ -1,2 +1 @@
 CONFIG_ZTEST=y
-CONFIG_ZTEST_NEW_API=y
diff --git a/scripts/tests/twister_blackbox/test_data/tests/dummy/agnostic/group2/prj.conf b/scripts/tests/twister_blackbox/test_data/tests/dummy/agnostic/group2/prj.conf
index 9228251051ec064..9467c2926896dd7 100644
--- a/scripts/tests/twister_blackbox/test_data/tests/dummy/agnostic/group2/prj.conf
+++ b/scripts/tests/twister_blackbox/test_data/tests/dummy/agnostic/group2/prj.conf
@@ -1,2 +1 @@
 CONFIG_ZTEST=y
-CONFIG_ZTEST_NEW_API=y
diff --git a/scripts/tests/twister_blackbox/test_data/tests/dummy/device/group/prj.conf b/scripts/tests/twister_blackbox/test_data/tests/dummy/device/group/prj.conf
index 9228251051ec064..9467c2926896dd7 100644
--- a/scripts/tests/twister_blackbox/test_data/tests/dummy/device/group/prj.conf
+++ b/scripts/tests/twister_blackbox/test_data/tests/dummy/device/group/prj.conf
@@ -1,2 +1 @@
 CONFIG_ZTEST=y
-CONFIG_ZTEST_NEW_API=y
diff --git a/subsys/testsuite/ztest/include/zephyr/ztest_test.h b/subsys/testsuite/ztest/include/zephyr/ztest_test.h
index a95a73ec4a05d76..aac7a7b2ad8807a 100644
--- a/subsys/testsuite/ztest/include/zephyr/ztest_test.h
+++ b/subsys/testsuite/ztest/include/zephyr/ztest_test.h
@@ -296,9 +296,6 @@ __syscall void __ztest_set_test_result(enum ztest_result new_result);
 __syscall void __ztest_set_test_phase(enum ztest_phase new_phase);
 #endif
 
-#ifndef ZTEST_UNITTEST
-#include <syscalls/ztest_test.h>
-#endif
 
 /**
  * @brief Fails the test if any of the registered tests did not run.