diff --git a/augment_transcriptome/README.md b/augment_transcriptome/README.md index 4dba527..c0ef59f 100644 --- a/augment_transcriptome/README.md +++ b/augment_transcriptome/README.md @@ -57,19 +57,21 @@ STRINGTIE_COMMAND: /mnt/cbis/home/yongshan/stringtie-2.2.1.Linux_x86_64/stringti GTF: /mnt/gtklab01/linglab/mmusculus_annotation_files/gencode.vM29.primary_assembly.annotation.gtf STRINGTIE_THREADS: 4 -# minimum junction coverage for Stringtie assembly: +# minimum junction coverage for Stringtie assembly JUNCS_CUTOFF: 20 -# --rf : assume stranded library fr-firststrand; --fr : assume stranded library fr-secondstrand: +# --rf : assume stranded library fr-firststrand; --fr : assume stranded library fr-secondstrand STRAND: rf -# alignments directory should contain all sample BAM & BAI files: +# alignments directory should contain all sample BAM & BAI files ALIGNMENTS_DIR: /mnt/gtklab01/linglab/tdp43/STAR/tdp43_nestin_ctx_e14/ ################### # MASTERLISTS PREP ################### +# depending on mouse or human, change ensembl version accordingly. Ignore the other ensembl version if not being used. mouse_ensembl_version: 106 +hsapiens_ensembl_version: 106 majiq_lsv_file_path: /mnt/cbis/home/yongshan/SpliCeAT/ds_detection/results/majiq_delta_psi/tdp43_nestin_ctx_e14/lsvs.txt leafcutter_cluster_sig_file_path: /mnt/cbis/home/yongshan/SpliCeAT/ds_detection/results/tdp43_nestin_ctx_e14_cluster_significance.txt leafcutter_effect_size_file_path: /mnt/cbis/home/yongshan/SpliCeAT/ds_detection/results/tdp43_nestin_ctx_e14_effect_sizes.txt