diff --git a/packages/virtual-list/src/vaadin-virtual-list-mixin.d.ts b/packages/virtual-list/src/vaadin-virtual-list-mixin.d.ts
index 54b7209ff1..d19c2be17a 100644
--- a/packages/virtual-list/src/vaadin-virtual-list-mixin.d.ts
+++ b/packages/virtual-list/src/vaadin-virtual-list-mixin.d.ts
@@ -53,6 +53,11 @@ export declare class VirtualListMixinClass<TItem = VirtualListDefaultItem> {
    */
   items: TItem[] | undefined;
 
+  /**
+   * A function that generates accessible names for virtual list items.
+   */
+  itemAccessibleNameGenerator?: (item: TItem) => string;
+
   /**
    * Scroll to a specific index in the virtual list.
    */
diff --git a/packages/virtual-list/src/vaadin-virtual-list-mixin.js b/packages/virtual-list/src/vaadin-virtual-list-mixin.js
index e5b3d427e2..0b58300970 100644
--- a/packages/virtual-list/src/vaadin-virtual-list-mixin.js
+++ b/packages/virtual-list/src/vaadin-virtual-list-mixin.js
@@ -36,13 +36,21 @@ export const VirtualListMixin = (superClass) =>
          */
         renderer: { type: Function, sync: true },
 
+        /**
+         * A function that generates accessible names for virtual list items.
+         */
+        itemAccessibleNameGenerator: {
+          type: Function,
+          sync: true,
+        },
+
         /** @private */
         __virtualizer: Object,
       };
     }
 
     static get observers() {
-      return ['__itemsOrRendererChanged(items, renderer, __virtualizer)'];
+      return ['__itemsOrRendererChanged(items, renderer, __virtualizer, itemAccessibleNameGenerator)'];
     }
 
     /**
@@ -84,6 +92,7 @@ export const VirtualListMixin = (superClass) =>
       this.addController(this.__overflowController);
 
       processTemplates(this);
+      this.__updateAria();
     }
 
     /** @protected */
@@ -116,18 +125,34 @@ export const VirtualListMixin = (superClass) =>
       return [...Array(count)].map(() => document.createElement('div'));
     }
 
+    /** @private */
+    __updateAria() {
+      this.role = 'list';
+    }
+
     /** @private */
     __updateElement(el, index) {
+      const item = this.items[index];
+      el.ariaSetSize = String(this.items.length);
+      el.ariaPosInSet = String(index + 1);
+      el.ariaLabel = this.itemAccessibleNameGenerator ? this.itemAccessibleNameGenerator(item) : null;
+      this.__updateElementRole(el);
+
       if (el.__renderer !== this.renderer) {
         el.__renderer = this.renderer;
         this.__clearRenderTargetContent(el);
       }
 
       if (this.renderer) {
-        this.renderer(el, this, { item: this.items[index], index });
+        this.renderer(el, this, { item, index });
       }
     }
 
+    /** @private */
+    __updateElementRole(el) {
+      el.role = 'listitem';
+    }
+
     /**
      * Clears the content of a render target.
      * @private
diff --git a/packages/virtual-list/test/typings/virtual-list.types.ts b/packages/virtual-list/test/typings/virtual-list.types.ts
index fc99602ab2..b70ee662ac 100644
--- a/packages/virtual-list/test/typings/virtual-list.types.ts
+++ b/packages/virtual-list/test/typings/virtual-list.types.ts
@@ -40,3 +40,5 @@ assertType<(index: number) => void>(virtualList.scrollToIndex);
 
 assertType<number>(virtualList.firstVisibleIndex);
 assertType<number>(virtualList.lastVisibleIndex);
+
+assertType<((item: TestVirtualListItem) => string) | undefined>(virtualList.itemAccessibleNameGenerator);
diff --git a/packages/virtual-list/test/virtual-list.common.js b/packages/virtual-list/test/virtual-list.common.js
index 79b3aae6f5..ebf27201e9 100644
--- a/packages/virtual-list/test/virtual-list.common.js
+++ b/packages/virtual-list/test/virtual-list.common.js
@@ -4,8 +4,9 @@ import { fixtureSync, nextFrame } from '@vaadin/testing-helpers';
 describe('virtual-list', () => {
   let list;
 
-  beforeEach(() => {
+  beforeEach(async () => {
     list = fixtureSync(`<vaadin-virtual-list></vaadin-virtual-list>`);
+    await nextFrame();
   });
 
   it('should have a default height', () => {
@@ -36,6 +37,10 @@ describe('virtual-list', () => {
     expect(flexBox.firstElementChild.offsetWidth).to.equal(flexBox.offsetWidth);
   });
 
+  it('should have role="list"', () => {
+    expect(list.role).to.equal('list');
+  });
+
   describe('with items', () => {
     beforeEach(async () => {
       const size = 100;
@@ -101,7 +106,7 @@ describe('virtual-list', () => {
     it('should have a last visible index', () => {
       const item = [...list.children].find((el) => el.textContent === `value-${list.lastVisibleIndex}`);
       const itemRect = item.getBoundingClientRect();
-      expect(list.getBoundingClientRect().bottom).to.be.within(itemRect.top, itemRect.bottom);
+      expect(list.getBoundingClientRect().bottom).to.be.within(itemRect.top, itemRect.bottom + 1);
     });
 
     it('should clear the old content after assigning a new renderer', () => {
@@ -126,6 +131,34 @@ describe('virtual-list', () => {
       expect(list.children[0].textContent.trim()).to.equal('bar');
     });
 
+    it('should have items with role="listitem"', () => {
+      expect(list.children[0].role).to.equal('listitem');
+    });
+
+    it('should assign aria-setsize and aria-posinset', () => {
+      list.scrollToIndex(list.items.length - 1);
+      const item = [...list.children].find((el) => el.textContent === `value-${list.lastVisibleIndex}`);
+      expect(item.ariaSetSize).to.equal('100');
+      expect(item.ariaPosInSet).to.equal('100');
+    });
+
+    describe('item accessible name generator', () => {
+      beforeEach(async () => {
+        list.itemAccessibleNameGenerator = (item) => `Accessible ${item.value}`;
+        await nextFrame();
+      });
+
+      it('should generate aria-label to the items', () => {
+        expect(list.children[0].ariaLabel).to.equal('Accessible value-0');
+      });
+
+      it('should remove aria-label from the items', async () => {
+        list.itemAccessibleNameGenerator = undefined;
+        await nextFrame();
+        expect(list.children[0].ariaLabel).to.be.null;
+      });
+    });
+
     describe('overflow attribute', () => {
       it('should set overflow attribute to "bottom" when scroll is at the beginning', () => {
         expect(list.getAttribute('overflow')).to.equal('bottom');