Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

SequenceFormatException: Midline #36

Open
awh082834 opened this issue Oct 20, 2022 · 0 comments
Open

SequenceFormatException: Midline #36

awh082834 opened this issue Oct 20, 2022 · 0 comments

Comments

@awh082834
Copy link

Hi, I have been trying to use Filtlong on some long read assemblers, mainly Flye and Raven. I have used it with Unicycler for a while now with no issues, however, there seems to be something with the formatting of the output file that is not accepted by Flye, Raven, or by Fastqc. When running through Fastqc it returns SequenceFormatException: Midline ' read sequence ' didnt start with '+'. I have also attached a link to a BioStars post I made a few weeks ago detailing what I was seeing when opening the fastq and going to the specified line. I've run this with different fastqs, some that were sequenced in house and some by others, so I don't think the original fastqs are an issue.

Thank you!

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant