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I am using filtlong extensively as pipeline component and really like it. For a fully fledged assembly pipeline I use it with the Illumina read option. Due to different library sizes the memory footprint and runtime is often different. Would it makes sense to add a option that takes a total base count and stops reading Illumina data to speed things up and also keep the memory footprint for a given species uniform? Could also be done by specifying genome size and a target coverage. Gut feeling, its enough to have 40-50x coverage for a diploid. Otherwise one has to run through the file beforehand.
Cheers,
Felix
The text was updated successfully, but these errors were encountered:
Hi Ryan,
I am using filtlong extensively as pipeline component and really like it. For a fully fledged assembly pipeline I use it with the Illumina read option. Due to different library sizes the memory footprint and runtime is often different. Would it makes sense to add a option that takes a total base count and stops reading Illumina data to speed things up and also keep the memory footprint for a given species uniform? Could also be done by specifying genome size and a target coverage. Gut feeling, its enough to have 40-50x coverage for a diploid. Otherwise one has to run through the file beforehand.
Cheers,
Felix
The text was updated successfully, but these errors were encountered: