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zE%N7EpAKN~kwQU@Y<5{{lS+hBcPe+X=JkqK6f@US;|Os(Tbht}EEV=a{>z0wv}anj zjdlv8Ysm1n Date: Wed, 3 Apr 2024 10:30:50 +0100 Subject: [PATCH 2/3] Creating 10.21105.joss.06332.crossref.xml --- joss.06332/10.21105.joss.06332.crossref.xml | 312 ++++++++++++++++++++ 1 file changed, 312 insertions(+) create mode 100644 joss.06332/10.21105.joss.06332.crossref.xml diff --git a/joss.06332/10.21105.joss.06332.crossref.xml b/joss.06332/10.21105.joss.06332.crossref.xml new file mode 100644 index 0000000000..5e7848ec16 --- /dev/null +++ b/joss.06332/10.21105.joss.06332.crossref.xml @@ -0,0 +1,312 @@ + + + + 20240403T093026-748913643e97b20ca046776f920c763bc678238d + 20240403093026 + + JOSS Admin + admin@theoj.org + + The Open Journal + + + + + Journal of Open Source Software + JOSS + 2475-9066 + + 10.21105/joss + https://joss.theoj.org + + + + + 04 + 2024 + + + 9 + + 96 + + + + Syn-CpG-Spacer: A Panel web app for synonymous recoding +of viral genomes with CpG dinucleotides + + + + Aleksander + Sulkowski + https://orcid.org/0000-0002-0624-428X + + + Clément + Bouton + https://orcid.org/0000-0001-9607-6533 + + + Chad + Swanson + https://orcid.org/0000-0002-6650-3634 + + + + 04 + 03 + 2024 + + + 6332 + + + 10.21105/joss.06332 + + + http://creativecommons.org/licenses/by/4.0/ + http://creativecommons.org/licenses/by/4.0/ + http://creativecommons.org/licenses/by/4.0/ + + + + Software archive + 10.5281/zenodo.10781374 + + + GitHub review issue + https://github.com/openjournals/joss-reviews/issues/6332 + + + + 10.21105/joss.06332 + https://joss.theoj.org/papers/10.21105/joss.06332 + + + https://joss.theoj.org/papers/10.21105/joss.06332.pdf + + + + + + Targeted restriction of viral gene expression +and replication by the ZAP antiviral system + Ficarelli + Annual Review of Virology + 1 + 8 + 10.1146/annurev-virology-091919-104213 + 2021 + Ficarelli, M., Neil, S. J. D., & +Swanson, C. M. (2021). Targeted restriction of viral gene expression and +replication by the ZAP antiviral system. Annual Review of Virology, +8(1), 265–283. +https://doi.org/10.1146/annurev-virology-091919-104213 + + + SSE: A nucleotide and amino acid sequence +analysis platform + Simmonds + BMC Research Notes + 1 + 5 + 10.1186/1756-0500-5-50 + 1756-0500 + 2012 + Simmonds, P. (2012). SSE: A +nucleotide and amino acid sequence analysis platform. BMC Research +Notes, 5(1), 1–10. +https://doi.org/10.1186/1756-0500-5-50 + + + SynMut: Designing synonymously mutated +sequences with different genomic signatures + Gu + 10.18129/B9.bioc.SynMut + 2023 + Gu, H., & Poon, L. L. M. (2023). +SynMut: Designing synonymously mutated sequences with different genomic +signatures (Version 1.16.0). Bioconductor version: Release (3.17). +https://doi.org/10.18129/B9.bioc.SynMut + + + Patterns of evolution and host gene mimicry +in influenza and other RNA viruses + Greenbaum + PLOS Pathogens + 6 + 4 + 10.1371/journal.ppat.1000079 + 1553-7374 + 2008 + Greenbaum, B. D., Levine, A. J., +Bhanot, G., & Rabadan, R. (2008). Patterns of evolution and host +gene mimicry in influenza and other RNA viruses. PLOS Pathogens, 4(6), +e1000079. +https://doi.org/10.1371/journal.ppat.1000079 + + + Attenuation of human respiratory viruses by +synonymous genome recoding + Le Nouën + Frontiers in Immunology + 10 + 10.3389/fimmu.2019.01250 + 1664-3224 + 2019 + Le Nouën, C., Collins, P. L., & +Buchholz, U. J. (2019). Attenuation of human respiratory viruses by +synonymous genome recoding. Frontiers in Immunology, 10. +https://doi.org/10.3389/fimmu.2019.01250 + + + Sequence determinants, function, and +evolution of CpG islands + Angeloni + Biochemical Society +Transactions + 3 + 49 + 10.1042/BST20200695 + 1470-8752 + 2021 + Angeloni, A., & Bogdanovic, O. +(2021). Sequence determinants, function, and evolution of CpG islands. +Biochemical Society Transactions, 49(3), 1109–1119. +https://doi.org/10.1042/BST20200695 + + + Dinucleotide biases in RNA viruses that +infect vertebrates or invertebrates + Forni + Microbiology Spectrum + 0 + 0 + 10.1128/spectrum.02529-23 + 2023 + Forni, D., Pozzoli, U., Cagliani, R., +Clerici, M., & Sironi, M. (2023). Dinucleotide biases in RNA viruses +that infect vertebrates or invertebrates. Microbiology Spectrum, 0(0), +e02529–23. +https://doi.org/10.1128/spectrum.02529-23 + + + CpG dinucleotide enrichment in the influenza +a virus genome as a live attenuated vaccine development +strategy + Sharp + PLOS Pathogens + 5 + 19 + 10.1371/journal.ppat.1011357 + 1553-7374 + 2023 + Sharp, C. P., Thompson, B. H., Nash, +T. J., Diebold, O., Pinto, R. M., Thorley, L., Lin, Y.-T., Sives, S., +Wise, H., Hendry, S. C., Grey, F., Vervelde, L., Simmonds, P., Digard, +P., & Gaunt, E. R. (2023). CpG dinucleotide enrichment in the +influenza a virus genome as a live attenuated vaccine development +strategy. PLOS Pathogens, 19(5), e1011357. +https://doi.org/10.1371/journal.ppat.1011357 + + + Elevation of CpG frequencies in influenza a +genome attenuates pathogenicity but enhances host response to +infection + Gaunt + eLife + 5 + 10.7554/eLife.12735 + 2050-084X + 2016 + Gaunt, E., Wise, H. M., Zhang, H., +Lee, L. N., Atkinson, N. J., Nicol, M. Q., Highton, A. J., Klenerman, +P., Beard, P. M., Dutia, B. M., Digard, P., & Simmonds, P. (2016). +Elevation of CpG frequencies in influenza a genome attenuates +pathogenicity but enhances host response to infection. eLife, 5, e12735. +https://doi.org/10.7554/eLife.12735 + + + Rational attenuation of RNA viruses with zinc +finger antiviral protein + Gonçalves-Carneiro + Nature Microbiology + 10 + 7 + 10.1038/s41564-022-01223-8 + 2058-5276 + 2022 + Gonçalves-Carneiro, D., Mastrocola, +E., Lei, X., DaSilva, J., Chan, Y. F., & Bieniasz, P. D. (2022). +Rational attenuation of RNA viruses with zinc finger antiviral protein. +Nature Microbiology, 7(10), 1558–1567. +https://doi.org/10.1038/s41564-022-01223-8 + + + Bokeh: Python library for interactive +visualization + Bokeh Development Team + 2024 + Bokeh Development Team. (2024). +Bokeh: Python library for interactive visualization (Version 3.3.4). +https://docs.bokeh.org/en/3.3.4/ + + + Biopython: Freely available python tools for +computational molecular biology and bioinformatics + Cock + Bioinformatics + 11 + 25 + 10.1093/bioinformatics/btp163 + 1367-4803 + 2009 + Cock, P. J. A., Antao, T., Chang, J. +T., Chapman, B. A., Cox, C. J., Dalke, A., Friedberg, I., Hamelryck, T., +Kauff, F., Wilczynski, B., & Hoon, M. J. L. de. (2009). Biopython: +Freely available python tools for computational molecular biology and +bioinformatics. Bioinformatics, 25(11), 1422–1423. +https://doi.org/10.1093/bioinformatics/btp163 + + + Progressive web apps + MDN Web Docs + 2023 + MDN Web Docs. (2023, October 25). +Progressive web apps. +https://developer.mozilla.org/en-US/docs/Web/Progressive_web_apps + + + Pyodide/pyodide: A python distribution for +the browser and node.js based on WebAssembly + The Pyodide development team + 10.5281/zenodo.8378122 + 2023 + The Pyodide development team. (2023). +Pyodide/pyodide: A python distribution for the browser and node.js based +on WebAssembly (Version 0.24.1). Zenodo. +https://doi.org/10.5281/zenodo.8378122 + + + Holoviz/panel: The powerful data exploration +& web app framework for python + Panel Development Team + 10.5281/zenodo.10563859 + 2024 + Panel Development Team. (2024). +Holoviz/panel: The powerful data exploration & web app framework for +python (Version v1.3.8). Zenodo. +https://doi.org/10.5281/zenodo.10563859 + + + + + + From c0aac339e58591e3ae7bb4e52bc5c57e441169da Mon Sep 17 00:00:00 2001 From: The Open Journals editorial robot <89919391+editorialbot@users.noreply.github.com> Date: Wed, 3 Apr 2024 10:30:50 +0100 Subject: [PATCH 3/3] Creating 10.21105.joss.06332.jats --- joss.06332/10.21105.joss.06332.jats | 493 ++++++++++++++++++++++++++++ 1 file changed, 493 insertions(+) create mode 100644 joss.06332/10.21105.joss.06332.jats diff --git a/joss.06332/10.21105.joss.06332.jats b/joss.06332/10.21105.joss.06332.jats new file mode 100644 index 0000000000..5abc4fee25 --- /dev/null +++ b/joss.06332/10.21105.joss.06332.jats @@ -0,0 +1,493 @@ + + +
+ + + + +Journal of Open Source Software +JOSS + +2475-9066 + +Open Journals + + + +6332 +10.21105/joss.06332 + +Syn-CpG-Spacer: A Panel web app for synonymous recoding +of viral genomes with CpG dinucleotides + + + +https://orcid.org/0000-0002-0624-428X + +Sulkowski +Aleksander + + +* + + +https://orcid.org/0000-0001-9607-6533 + +Bouton +Clément + + + + +https://orcid.org/0000-0002-6650-3634 + +Swanson +Chad + + + + + +Department of Infectious Diseases, King’s College London, +London, United Kingdom + + + + +* E-mail: + + +7 +11 +2023 + +9 +96 +6332 + +Authors of papers retain copyright and release the +work under a Creative Commons Attribution 4.0 International License (CC +BY 4.0) +2022 +The article authors + +Authors of papers retain copyright and release the work under +a Creative Commons Attribution 4.0 International License (CC BY +4.0) + + + +Python +virology +molecular biology +genetics +dinucleotide +CpG +genome recoding + + + + + + Summary +

Vertebrate genomes contain lower than expected frequencies of the + CpG dinucleotide. Consequently, many vertebrate viruses have evolved + to mimic this composition, possibly in order to evade host antiviral + defences + (Greenbaum + et al., 2008). For example, the antiviral protein ZAP binds + CpGs in viral single stranded RNA with specific spacing requirements + (Gonçalves-Carneiro + et al., 2022), though CpGs are also likely depleted in viral + genomes due to other selective pressures + (Forni + et al., 2023). Increasing CpG abundance by synonymous recoding + could facilitate attenuation of viruses without compromising their + epitope antigenicity by changing non-CpG codons to alternatives + containing CpG without changing the overall amino acid sequence + (Gonçalves-Carneiro + et al., 2022; + Le + Nouën et al., 2019; + Sharp + et al., 2023). There are three ways CpGs can be synonymously + introduced in codons: at positions 1-2 for arginine (e.g. AGA → CGA), + 2-3 for several amino acids (e.g. ACA → ACG), or in a 3-1 split + configuration, if a subsequent codon begins with a G (e.g. ATA-GCA → + ATC-GCA).

+

Syn-CpG-Spacer is a Python progressive web app (PWA) + (MDN + Web Docs, 2023) made with the Panel library + (Panel + Development Team, 2024) that allows for consistent recoding of + viral sequences and applying biologically relevant constraints. These + include setting a minimum gap between CpG’s, optimising for an average + CpG gap, protecting cis-acting regulatory signals from modification, + and modulating the A-content in the overall sequence. The app features + a sequence viewer made with the Bokeh library + (Bokeh + Development Team, 2024) that highlights CpG dinucleotides, + allowing for efficient analysis of the resulting distribution of CpGs. + This is complemented by a statistical data table. Utilising Biopython + (Cock + et al., 2009) modules, the user can load their sequence as a + FASTA file and download the outputs as an alignment in the same + format. As a PWA running on Pyodide + (The + Pyodide development team, 2023), the code is only executed in + the user’s browser and they can install the app onto their machine for + offline use.

+
+ + Statement of need +

There are currently no published tools that allow specifying the + spacing of CpG dinucleotides when synonymously recoding sequences. SSE + can recode a sequence to a defined CpG frequency, and has a graphical + interface + (Simmonds, + 2012). However, it cannot control the spacing of the CpGs. + Newer tools require the knowledge of R, such as SynMut + (Gu + & Poon, 2023). A problem with bioinformatics packages is a + high access barrier for users who are not familiar with programming + languages. Here, the installation and update processes are streamlined + thanks to the PWA approach.

+

With the current lack of CpG recoding tools, researchers may turn + to in-house solutions which can hamper the reproducibility of their + results, while also introducing the room for error. Syn-CpG-Spacer + makes it more efficient to synonymously recode a sequence compared to + doing so without any support tools.

+
+ + Research applications +

In recent years, there has been an increased research focus on + introducing CpGs into viral genomes as a mechanism to create live + attenuated virus vaccines, such as influenza A virus + (Gaunt + et al., 2016; + Sharp + et al., 2023). However, the mechanism of action for how CpGs + restrict the virus is unclear and it could be due to sensitising it to + antiviral proteins such as ZAP + (Ficarelli + et al., 2021) or other poorly characterised effects on viral + gene expression. This tool can be used to introduce CpGs into + different viral genomes with specific spacing to determine if this + attenuates the virus in vitro or in vivo and characterise the + mechanism of attenuation, which will aid the development of live + attenuated viral vaccines + (Le + Nouën et al., 2019).

+

Another potential application for the software is creation of CpG + islands, which are long stretches of DNA rich in CpG dinucleotides + that allow for epigenetic control of transcription. While most + vertebrate CpGs are methylated, and thus transcriptionally silent, the + DNA in CpG islands is hypomethylated, facilitating transcription + factor binding + (Angeloni + & Bogdanovic, 2021).

+
+ + Acknowledgements +

Aleksander Sulkowski is financially supported by the Association of + Clinical Pathologists (UK). Clément Bouton and Chad Swanson are + supported by the MRC grant MR/W018519/1.

+
+ + + + + + + FicarelliMattia + NeilStuart J. D. + SwansonChad M. + + Targeted restriction of viral gene expression and replication by the ZAP antiviral system + Annual Review of Virology + 2021 + 20230416 + 8 + 1 + https://doi.org/10.1146/annurev-virology-091919-104213 + 10.1146/annurev-virology-091919-104213 + 34129371 + 265 + 283 + + + + + + SimmondsPeter + + SSE: A nucleotide and amino acid sequence analysis platform + BMC Research Notes + 201212 + 20230820 + 5 + 1 + 1756-0500 + https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-5-50 + 10.1186/1756-0500-5-50 + 1 + 10 + + + + + + GuHaogao + PoonLeo L. M. + + SynMut: Designing synonymously mutated sequences with different genomic signatures + Bioconductor version: Release (3.17) + 2023 + 20230820 + https://bioconductor.org/packages/SynMut/ + 10.18129/B9.bioc.SynMut + + + + + + GreenbaumBenjamin D. + LevineArnold J. + BhanotGyan + RabadanRaul + + Patterns of evolution and host gene mimicry in influenza and other RNA viruses + PLOS Pathogens + 20080606 + 20230820 + 4 + 6 + 1553-7374 + https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000079 + 10.1371/journal.ppat.1000079 + e1000079 + + + + + + + Le NouënCyril + CollinsPeter L. + BuchholzUrsula J. + + Attenuation of human respiratory viruses by synonymous genome recoding + Frontiers in Immunology + 2019 + 20230820 + 10 + 1664-3224 + https://www.frontiersin.org/articles/10.3389/fimmu.2019.01250 + 10.3389/fimmu.2019.01250 + + + + + + AngeloniAllegra + BogdanovicOzren + + Sequence determinants, function, and evolution of CpG islands + Biochemical Society Transactions + 20210630 + 49 + 3 + 1470-8752 + 10.1042/BST20200695 + 34156435 + 1109 + 1119 + + + + + + ForniDiego + PozzoliUberto + CaglianiRachele + ClericiMario + SironiManuela + + Dinucleotide biases in RNA viruses that infect vertebrates or invertebrates + Microbiology Spectrum + 20231006 + 20231017 + 0 + 0 + https://journals.asm.org/doi/full/10.1128/spectrum.02529-23 + 10.1128/spectrum.02529-23 + e02529 + 23 + + + + + + SharpColin P. + ThompsonBeth H. + NashTessa J. + DieboldOla + PintoRute M. + ThorleyLuke + LinYao-Tang + SivesSamantha + WiseHelen + HendrySara Clohisey + GreyFinn + VerveldeLonneke + SimmondsPeter + DigardPaul + GauntEleanor R. + + CpG dinucleotide enrichment in the influenza a virus genome as a live attenuated vaccine development strategy + PLOS Pathogens + 20230505 + 20231017 + 19 + 5 + 1553-7374 + https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1011357 + 10.1371/journal.ppat.1011357 + e1011357 + + + + + + + GauntEleanor + WiseHelen M + ZhangHuayu + LeeLian N + AtkinsonNicky J + NicolMarlynne Quigg + HightonAndrew J + KlenermanPaul + BeardPhilippa M + DutiaBernadette M + DigardPaul + SimmondsPeter + + Elevation of CpG frequencies in influenza a genome attenuates pathogenicity but enhances host response to infection + eLife + + LipsitchMarc + + 20160216 + 20231017 + 5 + 2050-084X + https://doi.org/10.7554/eLife.12735 + 10.7554/eLife.12735 + e12735 + + + + + + + Gonçalves-CarneiroDaniel + MastrocolaEmily + LeiXiao + DaSilvaJustin + ChanYoke Fun + BieniaszPaul D. + + Rational attenuation of RNA viruses with zinc finger antiviral protein + Nature Microbiology + 202210 + 20231017 + 7 + 10 + 2058-5276 + https://www.nature.com/articles/s41564-022-01223-8 + 10.1038/s41564-022-01223-8 + 1558 + 1567 + + + + + + Bokeh Development Team + + Bokeh: Python library for interactive visualization + 202401 + 20240328 + https://docs.bokeh.org/en/3.3.4/ + + + + + + CockPeter J. A. + AntaoTiago + ChangJeffrey T. + ChapmanBrad A. + CoxCymon J. + DalkeAndrew + FriedbergIddo + HamelryckThomas + KauffFrank + WilczynskiBartek + HoonMichiel J. L. de + + Biopython: Freely available python tools for computational molecular biology and bioinformatics + Bioinformatics + 20090601 + 20240325 + 25 + 11 + 1367-4803 + https://doi.org/10.1093/bioinformatics/btp163 + 10.1093/bioinformatics/btp163 + 1422 + 1423 + + + + + + MDN Web Docs + + Progressive web apps + 20231025 + 20240325 + https://developer.mozilla.org/en-US/docs/Web/Progressive_web_apps + + + + + + The Pyodide development team + + Pyodide/pyodide: A python distribution for the browser and node.js based on WebAssembly + Zenodo + 20230925 + 20240325 + https://zenodo.org/records/8378122 + 10.5281/zenodo.8378122 + + + + + + Panel Development Team + + Holoviz/panel: The powerful data exploration & web app framework for python + Zenodo + 20240124 + 20240325 + https://zenodo.org/records/10563859 + 10.5281/zenodo.10563859 + + + + +