diff --git a/plugins/node/opentelemetry-instrumentation-mysql2/src/instrumentation.ts b/plugins/node/opentelemetry-instrumentation-mysql2/src/instrumentation.ts
index ef5562b115..ffbd9dc00b 100644
--- a/plugins/node/opentelemetry-instrumentation-mysql2/src/instrumentation.ts
+++ b/plugins/node/opentelemetry-instrumentation-mysql2/src/instrumentation.ts
@@ -154,8 +154,16 @@ export class MySQL2Instrumentation extends InstrumentationBase<MySQL2Instrumenta
           }
           span.end();
         });
-
-        if (typeof arguments[arguments.length - 1] !== 'function') {
+        function findCallback(_arguments: IArguments) {
+          for (let i=0; i<_arguments.length; i++) {
+            if (typeof _arguments[i] === 'function') {
+              return i;
+            }
+          }
+          return -1;
+        }
+        const cb = findCallback(arguments);
+        if (cb < 0) {
           if (typeof (query as any).onResult === 'function') {
             thisPlugin._wrap(
               query as any,
@@ -182,7 +190,7 @@ export class MySQL2Instrumentation extends InstrumentationBase<MySQL2Instrumenta
         } else {
           thisPlugin._wrap(
             arguments,
-            arguments.length - 1,
+            cb,
             thisPlugin._patchCallbackQuery(endSpan)
           );
         }
diff --git a/plugins/node/opentelemetry-instrumentation-mysql2/test/mysql.test.ts b/plugins/node/opentelemetry-instrumentation-mysql2/test/mysql.test.ts
index 517270f791..6afb10995f 100644
--- a/plugins/node/opentelemetry-instrumentation-mysql2/test/mysql.test.ts
+++ b/plugins/node/opentelemetry-instrumentation-mysql2/test/mysql.test.ts
@@ -722,6 +722,23 @@ describe('mysql2', () => {
       });
     });
 
+    it('should intercept pool.query(text: options, callback, null)', done => {
+      const span = provider.getTracer('default').startSpan('test span');
+      context.with(trace.setSpan(context.active(), span), () => {
+        const sql = 'SELECT 1+1 as solution';
+        // @ts-ignore
+        pool.query({ sql }, (err, res: mysqlTypes.RowDataPacket[]) => {
+          assert.ifError(err);
+          assert.ok(res);
+          assert.strictEqual(res[0].solution, 2);
+          const spans = memoryExporter.getFinishedSpans();
+          assert.strictEqual(spans.length, 1);
+          assertSpan(spans[0], sql);
+          done();
+        }, null);
+      });
+    });
+
     it('should intercept pool.query(text: string, values: [], callback)', done => {
       const span = provider.getTracer('default').startSpan('test span');
       context.with(trace.setSpan(context.active(), span), () => {