diff --git a/verifier/testdata/truststore/x509/tsa/test-mismatch/DigiCertTSARootSHA384.cer b/verifier/testdata/truststore/x509/tsa/test-mismatch/DigiCertTSARootSHA384.cer
new file mode 100644
index 00000000..99bcc84b
Binary files /dev/null and b/verifier/testdata/truststore/x509/tsa/test-mismatch/DigiCertTSARootSHA384.cer differ
diff --git a/verifier/testdata/truststore/x509/tsa/test-mismatch/wabbit-networks.io.crt b/verifier/testdata/truststore/x509/tsa/test-nonCA/wabbit-networks.io.crt
similarity index 100%
rename from verifier/testdata/truststore/x509/tsa/test-mismatch/wabbit-networks.io.crt
rename to verifier/testdata/truststore/x509/tsa/test-nonCA/wabbit-networks.io.crt
diff --git a/verifier/testdata/truststore/x509/tsa/test-nonSelfIssued/nonSelfIssued.crt b/verifier/testdata/truststore/x509/tsa/test-nonSelfIssued/nonSelfIssued.crt
new file mode 100644
index 00000000..6ec50052
Binary files /dev/null and b/verifier/testdata/truststore/x509/tsa/test-nonSelfIssued/nonSelfIssued.crt differ
diff --git a/verifier/truststore/truststore.go b/verifier/truststore/truststore.go
index 067f5e63..067372c1 100644
--- a/verifier/truststore/truststore.go
+++ b/verifier/truststore/truststore.go
@@ -15,6 +15,7 @@
 package truststore
 
 import (
+	"bytes"
 	"context"
 	"crypto/x509"
 	"errors"
@@ -106,6 +107,14 @@ func (trustStore *x509TrustStore) GetCertificates(ctx context.Context, storeType
 		if err := ValidateCertificates(certs); err != nil {
 			return nil, CertificateError{InnerError: err, Msg: fmt.Sprintf("failed to validate the trusted certificate %s in trust store %s of type %s", certFileName, namedStore, storeType)}
 		}
+		// we require TSA certificates in trust store to be root CA certificates
+		if storeType == TypeTSA {
+			for _, cert := range certs {
+				if err := isRootCACertificate(cert); err != nil {
+					return nil, CertificateError{InnerError: err, Msg: fmt.Sprintf("trusted certificate %s in trust store %s of type %s is invalid: %v", certFileName, namedStore, storeType, err.Error())}
+				}
+			}
+		}
 		certificates = append(certificates, certs...)
 	}
 	if len(certificates) < 1 {
@@ -137,3 +146,14 @@ func ValidateCertificates(certs []*x509.Certificate) error {
 func isValidStoreType(storeType Type) bool {
 	return slices.Contains(Types, storeType)
 }
+
+// isRootCACertificate returns nil if cert is a root CA certificate
+func isRootCACertificate(cert *x509.Certificate) error {
+	if err := cert.CheckSignatureFrom(cert); err != nil {
+		return fmt.Errorf("certificate with subject %q is not a root CA certificate: %w", cert.Subject, err)
+	}
+	if !bytes.Equal(cert.RawSubject, cert.RawIssuer) {
+		return fmt.Errorf("certificate with subject %q is not a root CA certificate: issuer (%s) and subject (%s) are not the same", cert.Subject, cert.Issuer, cert.Subject)
+	}
+	return nil
+}
diff --git a/verifier/truststore/truststore_test.go b/verifier/truststore/truststore_test.go
index 762553e5..3ee3c740 100644
--- a/verifier/truststore/truststore_test.go
+++ b/verifier/truststore/truststore_test.go
@@ -98,3 +98,31 @@ func TestValidateCertsWithLeafCert(t *testing.T) {
 		t.Fatalf("leaf cert in a trust store should return error %q, got: %v", expectedErr, err)
 	}
 }
+
+func TestGetCertFromValidTsaTrustStore(t *testing.T) {
+	// testing ../testdata/truststore/x509/tsa/test-nonCA/globalsignRoot.cer
+	_, err := trustStore.GetCertificates(context.Background(), "tsa", "test-timestamp")
+	if err != nil {
+		t.Fatalf("expected nil error, but got %s", err)
+	}
+}
+
+func TestGetCertFromInvalidTsaTrustStore(t *testing.T) {
+	t.Run("non CA certificate", func(t *testing.T) {
+		// testing ../testdata/truststore/x509/tsa/test-nonCA/wabbit-networks.io
+		expectedErrMsg := `trusted certificate wabbit-networks.io.crt in trust store test-nonCA of type tsa is invalid: certificate with subject "CN=wabbit-networks.io,O=Notary,L=Seattle,ST=WA,C=US" is not a root CA certificate: x509: invalid signature: parent certificate cannot sign this kind of certificate`
+		_, err := trustStore.GetCertificates(context.Background(), "tsa", "test-nonCA")
+		if err == nil || err.Error() != expectedErrMsg {
+			t.Fatalf("expected error: %s, but got %s", expectedErrMsg, err)
+		}
+	})
+
+	t.Run("not self-issued", func(t *testing.T) {
+		//testing ../testdata/truststore/x509/tsa/test-nonSelfIssued/nonSelfIssued.crt
+		expectedErrMsg := `trusted certificate nonSelfIssued.crt in trust store test-nonSelfIssued of type tsa is invalid: certificate with subject "CN=Notation Test Revokable RSA Chain Cert 2,O=Notary,L=Seattle,ST=WA,C=US" is not a root CA certificate: issuer (CN=Notation Test Revokable RSA Chain Cert Root,O=Notary,L=Seattle,ST=WA,C=US) and subject (CN=Notation Test Revokable RSA Chain Cert 2,O=Notary,L=Seattle,ST=WA,C=US) are not the same`
+		_, err := trustStore.GetCertificates(context.Background(), "tsa", "test-nonSelfIssued")
+		if err == nil || err.Error() != expectedErrMsg {
+			t.Fatalf("expected error: %s, but got %s", expectedErrMsg, err)
+		}
+	})
+}