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Is it possible to use a Bonito trained or fine-tuned model with dorado duplex basecalling. #395

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mark-sor2 opened this issue Jun 17, 2024 · 0 comments

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@mark-sor2
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          Is it possible to use a Bonito trained or fine-tuned model with dorado duplex basecalling.

I'm getting next error in both cases:

dorado duplex ./dna_DordaoModel_fineTuned/ ./myPod5FIles/ > ./basecalledData/res.bam

[2024-06-17 12:39:39.076] [info] Running: "duplex" "./dna_DordaoModel_fineTuned/" "./myPod5FIles/ "
[2024-06-17 12:39:39.078] [info] > No duplex pairs file provided, pairing will be performed automatically
[2024-06-17 12:39:40.559] [error] finalise() not called on a HtsFile.
[2024-06-17 12:39:40.569] [error] Could not find information on simplex model: dna_DordaoModel_fineTuned

Any help would be great.

By the way, to export my trained model I used :
bonito export --output ./dna_DordaoModel_fineTuned/ --config trainedModel/dna_model_fineTune/config.toml trainedModel/dna_model_fineTune/

Originally posted by @mark-sor2 in #304 (comment)

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