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FNE_gedde_Norway_samples.txt
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FNE_gedde_Norway_samples.txt
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sampl_no,Fixated,Type_smpl,buffer_added,protocol,Enzyme_addition,Incubation_temp_and_period,lat,lon,date
1,ATL,Blank,Tilsette ATL som vanlig referanse,Qiagen etterfoelgende samme protokoll som vanlig,Add ProtK,Incubate at 56C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
2,ATL,Blank,Tilsette ATL som vanlig referanse,Qiagen etterfoelgende samme protokoll som vanlig,Add ProtK,Incubate at 56C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
3,ATL,Blank,Tilsette ATL som vanlig referanse,Qiagen etterfoelgende samme protokoll som vanlig,Add ProtK,Incubate at 56C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
4,ATL,Boette - konsentrat,Tilsette ATL som vanlig referanse,Qiagen etterfoelgende samme protokoll som vanlig,Add ProtK,Incubate at 56C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
5,ATL,Boette - konsentrat,Tilsette ATL som vanlig referanse,Qiagen etterfoelgende samme protokoll som vanlig,Add ProtK,Incubate at 56C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
6,ATL,Boette - konsentrat,Tilsette ATL som vanlig referanse,Qiagen etterfoelgende samme protokoll som vanlig,Add ProtK,Incubate at 56C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
7,ATL,Boette - konsentrat,Tilsette ATL som vanlig ekstraheres uten oppvarming,Qiagen uten oppvarming,Add ProtK,Incubate at 22C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
8,ATL,Boette - konsentrat,Tilsette ATL som vanlig ekstraheres uten oppvarming,Qiagen uten oppvarming,Add ProtK,Incubate at 22C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
9,ATL,Boette - konsentrat,Tilsette ATL som vanlig ekstraheres uten oppvarming,Qiagen uten oppvarming,Add ProtK,Incubate at 22C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
10,0.5 RNA,Boette - konsentrat,Tilsette 1 ml RNAgem hoeyt volum RNAgem ,RNAgem tilsatt 1 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
11,0.5 RNA,Boette - konsentrat,Tilsette 1 ml RNAgem hoeyt volum RNAgem ,RNAgem tilsatt 1 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
12,0.5 RNA,Boette - konsentrat,Tilsette 1 ml RNAgem hoeyt volum RNAgem ,RNAgem tilsatt 1 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
13,1.0 RNA,Boette - konsentrat,Tilsette 0.5 ml RNAgem lavt volum RNAgem,RNAgem tilsamm 0.5 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
14,1.0 RNA,Boette - konsentrat,Tilsette 0.5 ml RNAgem lavt volum RNAgem,RNAgem tilsamm 0.5 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
15,1.0 RNA,Boette - konsentrat,Tilsette 0.5 ml RNAgem lavt volum RNAgem,RNAgem tilsamm 0.5 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
16,ATL,1/100,Tilsette ATL som vanlig referanse,Qiagen etterfoelgende samme protokoll som vanlig,Add ProtK,Incubate at 56C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
17,ATL,1/100,Tilsette ATL som vanlig referanse,Qiagen etterfoelgende samme protokoll som vanlig,Add ProtK,Incubate at 56C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
18,ATL,1/100,Tilsette ATL som vanlig referanse,Qiagen etterfoelgende samme protokoll som vanlig,Add ProtK,Incubate at 56C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
19,ATL,1/100,Tilsette ATL som vanlig ekstraheres uten oppvarming,Qiagen uten oppvarming,Add ProtK,Incubate at 22C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
20,ATL,1/100,Tilsette ATL som vanlig ekstraheres uten oppvarming,Qiagen uten oppvarming,Add ProtK,Incubate at 22C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
21,ATL,1/100,Tilsette ATL som vanlig ekstraheres uten oppvarming,Qiagen uten oppvarming,Add ProtK,Incubate at 22C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
22,0.5 RNA,1/100,Tilsette 1 ml RNAgem hoeyt volum RNAgem ,RNAgem tilsatt 1 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
23,0.5 RNA,1/100,Tilsette 1 ml RNAgem hoeyt volum RNAgem ,RNAgem tilsatt 1 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
24,0.5 RNA,1/100,Tilsette 1 ml RNAgem hoeyt volum RNAgem ,RNAgem tilsatt 1 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
25,1.0 RNA,1/100,Tilsette 0.5 ml RNAgem lavt volum RNAgem,RNAgem tilsamm 0.5 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
26,1.0 RNA,1/100,Tilsette 0.5 ml RNAgem lavt volum RNAgem,RNAgem tilsamm 0.5 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
27,1.0 RNA,1/100,Tilsette 0.5 ml RNAgem lavt volum RNAgem,RNAgem tilsamm 0.5 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
30,ATL,1/1000,Tilsette ATL som vanlig referanse,Qiagen etterfoelgende samme protokoll som vanlig,Add ProtK,Incubate at 56C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
31,ATL,1/1000,Tilsette ATL som vanlig referanse,Qiagen etterfoelgende samme protokoll som vanlig,Add ProtK,Incubate at 56C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
32,ATL,1/1000,Tilsette ATL som vanlig referanse,Qiagen etterfoelgende samme protokoll som vanlig,Add ProtK,Incubate at 56C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
33,ATL,1/1000,Tilsette ATL som vanlig ekstraheres uten oppvarming,Qiagen uten oppvarming,Add ProtK,Incubate at 22C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
34,ATL,1/1000,Tilsette ATL som vanlig ekstraheres uten oppvarming,Qiagen uten oppvarming,Add ProtK,Incubate at 22C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
35,ATL,1/1000,Tilsette ATL som vanlig ekstraheres uten oppvarming,Qiagen uten oppvarming,Add ProtK,Incubate at 22C for minimum 4 hours,59.2523911,10.3321997,28.04.2021
36,0.5 RNA,1/1000,Tilsette 1 ml RNAgem hoeyt volum RNAgem ,RNAgem tilsatt 1 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
37,0.5 RNA,1/1000,Tilsette 1 ml RNAgem hoeyt volum RNAgem ,RNAgem tilsatt 1 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
38,0.5 RNA,1/1000,Tilsette 1 ml RNAgem hoeyt volum RNAgem ,RNAgem tilsatt 1 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
39,1.0 RNA,1/1000,Tilsette 0.5 ml RNAgem lavt volum RNAgem,RNAgem tilsamm 0.5 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
40,1.0 RNA,1/1000,Tilsette 0.5 ml RNAgem lavt volum RNAgem,RNAgem tilsamm 0.5 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
41,1.0 RNA,1/1000,Tilsette 0.5 ml RNAgem lavt volum RNAgem,RNAgem tilsamm 0.5 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
13.02,1.0 RNA,Boette - konsentrat,Tilsette 0.5 ml RNAgem lavt volum RNAgem,RNAgem tilsamm 0.5 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021
14.02,1.0 RNA,Boette - konsentrat,Tilsette 0.5 ml RNAgem lavt volum RNAgem,RNAgem tilsamm 0.5 ml Volcano lysis solution se protokoll under start paa Step 2,None,Incubate at 75C for minimum 15 minutes,59.2523911,10.3321997,28.04.2021