This is the repository hosting the MoBIE project that contains the tomography data described in:
A high-throughput electron tomography workflow reveals over-elongated centrioles in relapsed/refractory multiple myeloma
Dittrich et al. (2022) Cell Reports Methods
Visualizing the organelle structure in patient-derived tissues at nanoscale can provide insights into numerous pathologies, but existing tools do not allow for analysis of sufficient numbers of cells at the required resolution. Here, by applying a semi-automated high-throughput electron tomography strategy, we quantify structural abnormalities of centrioles in human bone-marrow-derived cells at high numbers and share our data with the cell biological community to illustrate its utility as a tool for structural organelle analysis.
In order to load one of the data-sets in MoBIE, you need to install the MoBIE Fiji Plugin, select Plugins->MoBIE->Open->Open MoBIE Project and enter https://github.com/mobie/centriole-tomo-examples. Or select it from the Open published project menu entry.
For more details on MoBIE and the MoBIE data format, visit https://mobie.github.io/.