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I'm trying to generate a custom genome using the provided sjdb file by running
this:
"$star_Path --runMode genomeGenerate --genomeDir $star_genome_dir
--genomeFastaFiles $genomeFastaFiles --runThreadN 8 --sjdbFileChrStartEnd
$sjdbOverhangFile --sjdbOverhang 91"
I'm using the mm9.fa and the Mus_musculus.NCBIM37.66.gtf.sjdb file both
provided under genome downloads, however this is the output:
EXITING because of FATAL error, the sjdb chromosome Y is not found among the
genomic chromosomes
SOLUTION: fix your file
sjdbFileChrStartEnd=...../Mus_musculus.NCBIM37.66.gtf.sjdb_OG.txt at line #1
What is wrong?
Olivier
Original issue reported on code.google.com by [email protected] on 12 Sep 2013 at 11:52
The text was updated successfully, but these errors were encountered:
Hi Olivier,
this issue is caused by the different naming of chromosomes in mm9.fa
(chromosomes' names start with 'chr') and Mus_musculus.NCBIM37.66.gtf.sjdb
(chromosomes' names do not contain 'chr'). You would need to synchronize the
naming of chromosomes in two files.
Please ask your questions at https://groups.google.com/forum/#!forum/rna-star
for a quicker response.
Cheers
Alex
Original issue reported on code.google.com by
[email protected]
on 12 Sep 2013 at 11:52The text was updated successfully, but these errors were encountered: