diff --git a/.kokoro/requirements.txt b/.kokoro/requirements.txt
index 2e425b36..6b481ff4 100644
--- a/.kokoro/requirements.txt
+++ b/.kokoro/requirements.txt
@@ -627,9 +627,9 @@ tomli==2.2.1 \
     --hash=sha256:ece47d672db52ac607a3d9599a9d48dcb2f2f735c6c2d1f34130085bb12b112a \
     --hash=sha256:f4039b9cbc3048b2416cc57ab3bda989a6fcf9b36cf8937f01a6e731b64f80d7
     # via -r requirements.in
-twine==5.1.1 \
-    --hash=sha256:215dbe7b4b94c2c50a7315c0275d2258399280fbb7d04182c7e55e24b5f93997 \
-    --hash=sha256:9aa0825139c02b3434d913545c7b847a21c835e11597f5255842d457da2322db
+twine==6.0.1 \
+    --hash=sha256:36158b09df5406e1c9c1fb8edb24fc2be387709443e7376689b938531582ee27 \
+    --hash=sha256:9c6025b203b51521d53e200f4a08b116dee7500a38591668c6a6033117bdc218
     # via -r requirements.in
 typing-extensions==4.12.2 \
     --hash=sha256:04e5ca0351e0f3f85c6853954072df659d0d13fac324d0072316b67d7794700d \