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Add species Neisseria gonorrhoeae #291

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fwa93 opened this issue Apr 25, 2024 · 4 comments
Open

Add species Neisseria gonorrhoeae #291

fwa93 opened this issue Apr 25, 2024 · 4 comments
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@fwa93
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fwa93 commented Apr 25, 2024

Add Neisseria gonorrhoeae to JASEN.

@mhkc
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mhkc commented May 15, 2024

Thanks for opening an issue. What types of analysis methods would you be interested in and think are relevant for Neisseria gonorrhoeae?

@mhkc mhkc added the could label May 15, 2024
@fwa93
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fwa93 commented May 15, 2024

Hej,
Daniel G som arbetar på WHO-labbet här i Ö arbetar mycket med den här arten och tipsar om dessa delar.

Clean reads - remove adaptors, low quality bases and reads (Trimmomatic)
Species confirmation - k-mer analysis (Kraken2, centrifuge or similar)
De novo assembly - SPAdes assembly and compilation of stats (number of contigs, size, etc)
Annotation - Prokka
Genotypning - MLST + NG-STAR + NG-STAR CC (pyngoST https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10868605/)
Resistome - Vi har en uppdaterad databas för alla gener som behöver karakteriseras (ARIBA https://github.com/sanger-pathogens/ariba)

Snippy - mappa mot referens
SNP-distanser - Matrix (snp-dist)
IQtree - ML GTR+G4 model
Rekombinationer - Identifiera och maska rekombinationer (gubbins)

Pan-genome - prokka på assembly+Roary
något sådant är det väl, vi kan snacka mer om du vill

@mhkc
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mhkc commented May 15, 2024

Toppen!

Vi får ta upp denna arten på ett GMS mikro möte och om någon vill ta på sig att utveckla en analys för arten. Tror du Daniel skulle vara intresserad i och med att han arbetar med arten?

@samuell
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samuell commented May 21, 2024

Hi, can just add that we in Stockholm are also interested in having Neisseria added (though we need like 2-3 other organisms too, so would be awesome if someone else is able to take on some of them :) ).

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