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Set temp directory for mutect2
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seppinho committed Mar 4, 2024
1 parent 3986333 commit 4653c99
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2 changes: 2 additions & 0 deletions modules/local/mutect2.nf
Original file line number Diff line number Diff line change
Expand Up @@ -20,13 +20,15 @@ process MUTECT2 {
-callable-depth 6 \
--native-pair-hmm-threads ${task.cpus} \
--max-reads-per-alignment-start 0 \
--tmp-dir \$PWD \
-I ${bam_file} \
-O raw.vcf.gz
gatk FilterMutectCalls \
-R ${reference} \
--min-reads-per-strand 2 \
-V raw.vcf.gz \
--tmp-dir \$PWD \
-O ${bam_file.baseName}.vcf.gz
bcftools norm \
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