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# mtDNA-Server 2 | ||
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[![mtdna-server-2](https://github.com/genepi/mtdna-server-2/actions/workflows/run-tests.yml/badge.svg)](https://github.com/genepi/mtdna-server-2/actions/workflows/run-tests.yml) | ||
[![nf-test](https://img.shields.io/badge/tested_with-nf--test-337ab7.svg)](https://github.com/askimed/nf-test) | ||
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Welcome to mtdna-Server 2. This repository includes the mtDNA-Server-2 Workflow ported to Nextflow. | ||
Welcome to mtdna-Server 2. This repository includes the mtDNA-Server-2 Workflow ported to Nextflow. | ||
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## Access | ||
mtDNA-Server 2 is hosted as a service on our [mitoverse](https://mitoverse.i-med.ac.at/) platform. | ||
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mtDNA-Server 2 is hosted as a service on our [mitoverse](https://mitoverse.i-med.ac.at/) platform. | ||
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## Documentation | ||
Documentation can be accessed [here](https://mitoverse.readthedocs.io). | ||
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Documentation can be accessed [here](https://mitoverse.readthedocs.io/mtdna-server/mtdna-server/). | ||
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## Local Installation | ||
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### Run mtDNA-Server 2 workflow | ||
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#### Requirements | ||
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- Docker or Singularity | ||
- Java 14 | ||
- Nextflow: | ||
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``` | ||
curl -s https://get.nextflow.io | bash | ||
``` | ||
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#### Execution | ||
#### Installation | ||
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Build Docker image: | ||
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``` | ||
docker build -t genepi/mtdna-server-2 . # don't ingore the dot here | ||
``` | ||
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#### Execution | ||
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``` | ||
nextflow run main.nf -c tests/test_single_bam.config -profile development | ||
``` | ||
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#### Installation | ||
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- Install cloudgene3: `curl -s install.cloudgene.io | bash -s 3.0.0-beta4` | ||
- Download latest source code zip file from releases | ||
- Install mtDNA-Server 2 workflow: `./cloudgene install https://github.com/genepi/mtdna-server-2/archive/refs/tags/v2.0.0-beta2.zip` | ||
- Start cloudgene server: `./cloudgene server` | ||
- Open [http://localhost:8082](http://localhost:8082) | ||
- Login with default admin account: username `admin` and password `admin1978` | ||
- mtDNA-Server-2 can be tested with the following [test file](https://github.com/genepi/mtdna-server-2/raw/main/tests/data/bam/HG00096.mapped.ILLUMINA.bwa.GBR.low_coverage.20101123.bam)) | ||
- mtDNA-Server-2 can be tested with the following [test file](https://github.com/genepi/mtdna-server-2/raw/main/tests/data/bam/HG00096.mapped.ILLUMINA.bwa.GBR.low_coverage.20101123.bam) | ||
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## Publication | ||
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Weissensteiner H*, Forer L*, Fuchsberger C, Schöpf B, Kloss-Brandstätter A, Specht G, Kronenberg F, Schönherr S: mtDNA-Server: next-generation sequencing data analysis of human mitochondrial DNA in the cloud. Nucleic Acids Res. 44:W64-9, 2016. PMID: [27084948](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987870/) | ||
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## Contact | ||
Institute of Genetic Epidemiology, Medical University of Innsbruck | ||
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This software was developed at the [Institute of Genetic Epidemiology](https://genepi.i-med.ac.at/), [Medical University of Innsbruck](https://i-med.ac.at/) | ||
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![](https://avatars2.githubusercontent.com/u/1942824?s=30) [Sebastian Schoenherr](mailto:[email protected]) ([@seppinho](https://twitter.com/seppinho)) | ||
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![](https://avatars2.githubusercontent.com/u/1931865?s=30) [Hansi Weissensteiner](mailto:[email protected]) ([@whansi](https://twitter.com/whansi)) | ||
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![](https://avatars2.githubusercontent.com/u/210220?s=30) [Lukas Forer](mailto:[email protected]) ([@lukfor](https://twitter.com/lukfor)) | ||
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## License | ||
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MIT Licensed. |