Informative more than funny...
Michael P. Lockhart-Cairns - University of Manchester John Smith - DLS
Cryo-EM & Tomography of Matrix Proteins and their Niche
Abstracts are hard to write. That is why we write this at the end.
Cryo-EM is a method that has recently won the Nobel Prize for Chemistry. It is used to look at individual protein molecules. The work by Richard Henderson was vital to the atomic resolution of structures generated Henderson et al (2004). Dubouchet et al (2001) were instrumental for the freezing of single particle proteins using liquid ethane.
Molecular dynamic simulations were used to identify the most reasonable conformation of the protein withregards to the volume from EM.
We use CNS to do the MD simulations using torsion angle MD, although using GPU accelerated GROMAS (Lindahl et al 2005) with REMD may be better.
It was possible to accurately obtain the structure of BMPER using the MD and cryo-EM
The structure of BMPER was obtained using a combination of cryo-EM and MD with a resolution of 3.8 Angstroms.
Add figures here. This is a draft.
We would like to thank our EU funding body. RIP (?)