diff --git a/easybuild/easyconfigs/c/Cassiopeia/Cassiopeia-2.0.0-foss-2023a.eb b/easybuild/easyconfigs/c/Cassiopeia/Cassiopeia-2.0.0-foss-2023a.eb
new file mode 100644
index 00000000000..69b70834b9b
--- /dev/null
+++ b/easybuild/easyconfigs/c/Cassiopeia/Cassiopeia-2.0.0-foss-2023a.eb
@@ -0,0 +1,132 @@
+easyblock = 'PythonBundle'
+
+name = 'Cassiopeia'
+version = '2.0.0'
+
+homepage = 'https://github.com/YosefLab/Cassiopeia'
+description = """A Package for Cas9-Enabled Single Cell Lineage Tracing Tree Reconstruction."""
+
+toolchain = {'name': 'foss', 'version': '2023a'}
+
+builddependencies = [
+    ('CMake', '3.26.3'),
+    ('poetry', '1.5.1'),
+]
+
+dependencies = [
+    ('Python', '3.11.3'),
+    ('Python-bundle-PyPI', '2023.06'),
+    ('Biopython', '1.83'),
+    ('scikit-build', '0.17.6'),
+    ('bokeh', '3.2.2'),
+    ('ETE', '3.1.3'),
+    ('matplotlib', '3.7.2'),
+    ('networkx', '3.1'),
+    ('numba', '0.58.1'),
+    ('Pysam', '0.22.0'),
+    ('PyYAML', '6.0'),
+    ('typing-extensions', '4.9.0'),
+    ('tqdm', '4.66.1'),
+    ('hatchling', '1.18.0'),
+    ('BeautifulSoup', '4.12.2'),
+    ('statsmodels', '0.14.1'),
+    ('Seaborn', '0.13.2'),
+    ('IPython', '8.14.0'),
+    ('PyZMQ', '25.1.1'),
+]
+
+use_pip = True
+sanity_pip_check = True
+
+exts_list = [
+    ('hatch_jupyter_builder', '0.9.1', {
+        'checksums': ['79278198d124c646b799c5e8dca8504aed9dcaaa88d071a09eb0b5c2009a58ad'],
+    }),
+    ('hatch_nodejs_version', '0.3.2', {
+        'checksums': ['8a7828d817b71e50bbbbb01c9bfc0b329657b7900c56846489b9c958de15b54c'],
+    }),
+    ('deprecation', '2.1.0', {
+        'checksums': ['72b3bde64e5d778694b0cf68178aed03d15e15477116add3fb773e581f9518ff'],
+    }),
+    ('jupyter_packaging', '0.12.3', {
+        'checksums': ['9d9b2b63b97ffd67a8bc5391c32a421bc415b264a32c99e4d8d8dd31daae9cf4'],
+    }),
+    ('jupyter_client', '8.6.1', {
+        'checksums': ['e842515e2bab8e19186d89fdfea7abd15e39dd581f94e399f00e2af5a1652d3f'],
+    }),
+    ('widgetsnbextension', '4.0.10', {
+        'checksums': ['64196c5ff3b9a9183a8e699a4227fb0b7002f252c814098e66c4d1cd0644688f'],
+    }),
+    ('jupyterlab_widgets', '3.0.10', {
+        'checksums': ['04f2ac04976727e4f9d0fa91cdc2f1ab860f965e504c29dbd6a65c882c9d04c0'],
+    }),
+    ('comm', '0.2.2', {
+        'checksums': ['3fd7a84065306e07bea1773df6eb8282de51ba82f77c72f9c85716ab11fe980e'],
+    }),
+    ('fastjsonschema', '2.19.1', {
+        'checksums': ['e3126a94bdc4623d3de4485f8d468a12f02a67921315ddc87836d6e456dc789d'],
+    }),
+    ('ipywidgets', '8.1.2', {
+        'checksums': ['d0b9b41e49bae926a866e613a39b0f0097745d2b9f1f3dd406641b4a57ec42c9'],
+    }),
+    ('bleach', '6.1.0', {
+        'checksums': ['0a31f1837963c41d46bbf1331b8778e1308ea0791db03cc4e7357b97cf42a8fe'],
+    }),
+    ('defusedxml', '0.7.1', {
+        'checksums': ['1bb3032db185915b62d7c6209c5a8792be6a32ab2fedacc84e01b52c51aa3e69'],
+    }),
+    ('jupyter_core', '5.7.2', {
+        'checksums': ['aa5f8d32bbf6b431ac830496da7392035d6f61b4f54872f15c4bd2a9c3f536d9'],
+    }),
+    ('jupyterlab_pygments', '0.3.0', {
+        'checksums': ['721aca4d9029252b11cfa9d185e5b5af4d54772bb8072f9b7036f4170054d35d'],
+    }),
+    ('mistune', '3.0.2', {
+        'checksums': ['fc7f93ded930c92394ef2cb6f04a8aabab4117a91449e72dcc8dfa646a508be8'],
+    }),
+    ('nbclient', '0.10.0', {
+        'checksums': ['4b3f1b7dba531e498449c4db4f53da339c91d449dc11e9af3a43b4eb5c5abb09'],
+    }),
+    ('pandocfilters', '1.5.1', {
+        'checksums': ['002b4a555ee4ebc03f8b66307e287fa492e4a77b4ea14d3f934328297bb4939e'],
+    }),
+    ('tinycss2', '1.2.1', {
+        'checksums': ['8cff3a8f066c2ec677c06dbc7b45619804a6938478d9d73c284b29d14ecb0627'],
+    }),
+    ('traitlets', '5.14.2', {
+        'checksums': ['8cdd83c040dab7d1dee822678e5f5d100b514f7b72b01615b26fc5718916fdf9'],
+    }),
+    ('shortuuid', '1.0.13', {
+        'checksums': ['3bb9cf07f606260584b1df46399c0b87dd84773e7b25912b7e391e30797c5e72'],
+    }),
+    ('ngs-tools', '1.8.5', {
+        'checksums': ['380e236a101c5b1ac3c0fcdbcc908a210179b6ef2a93fbea9f4eb0ec2edc1de0'],
+    }),
+    ('nbformat', '5.10.3', {
+        'checksums': ['60ed5e910ef7c6264b87d644f276b1b49e24011930deef54605188ddeb211685'],
+    }),
+    ('nbconvert', '7.16.3', {
+        'checksums': ['a6733b78ce3d47c3f85e504998495b07e6ea9cf9bf6ec1c98dda63ec6ad19142'],
+    }),
+    ('itolapi', '4.1.4', {
+        'checksums': ['68e87ba51d209da556b0e373b3b0456b644a1a732c193fedbd7785ff37b6a2cb'],
+    }),
+    ('hits', '0.4.0', {
+        'checksums': ['743bfc1b56ab8fcf9fefacfcad4c1f23e9bafec1b42225709dbe097c8e669383'],
+    }),
+    ('Levenshtein', '0.22.0', {
+        'modulename': False,
+        'checksums': ['86d285d770551cb648d4fcfe5243449a479e694e56b65272dc6cbda879012051'],
+    }),
+    ('python-Levenshtein', '0.22.0', {
+        'modulename': 'Levenshtein',
+        'checksums': ['6f8e2216fbd4610ccfa1bab2e0052fb23de34c2333c192de08150eeb99717702'],
+    }),
+    (name, version, {
+        'source_urls': ['https://github.com/YosefLab/Cassiopeia/archive/'],
+        'sources': [{'download_filename': '%(version)s.tar.gz', 'filename': '%(name)s-%(version)s.tar.gz'}],
+        'checksums': ['28ae7495152683f5733222255056a1e7b2f7a51e1cee2f23f0d9f8ae5f4c3742'],
+    }),
+]
+
+moduleclass = 'bio'