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marker controlled Watershed #17

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haesleinhuepf opened this issue Sep 24, 2020 · 4 comments
Open

marker controlled Watershed #17

haesleinhuepf opened this issue Sep 24, 2020 · 4 comments
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enhancement New feature or request

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@haesleinhuepf
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Suggested by @pr4deepr

'''
Marker controlled watershed from MorpholibJ
If I am using CLIJx to generate seeds (labelling) and binary images (mask), then if I have edges image I can use these images for segmenting cells using Marker Controlled Watershed.
'''

See also:
https://github.com/clij/assistant/issues/14

@haesleinhuepf
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Hey @pr4deepr ,

have you tried
https://clij.github.io/clij2-docs/reference_seededWatershed

?

Thanks!

@haesleinhuepf haesleinhuepf added enhancement New feature or request help wanted Extra attention is needed and removed help wanted Extra attention is needed labels Sep 24, 2020
@haesleinhuepf haesleinhuepf changed the title wishlist: marker controlled Watershed marker controlled Watershed Sep 24, 2020
@pr4deepr
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I just tried it.

  • Top row is seeded watershed: The input image from CLIJx assistant, followed by labelling workflow ALX and then seeded watershed in CLIJx.
  • Bottom row is marker controlled watershed: the edges image (Canny-Deriche) and binary image with same threshold specified as in CLIJx assistant. The final labelled image is on the right.

The labelling wasn't optimised as much, but this demonstrates the differences.

image

@haesleinhuepf
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Awesome! I can see the issue now. Does it also fail in 2D? Can you share the dataset and the script which generates both results? That would make it easy for me to trace it down in more detail.

@pr4deepr
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I haven't tried 2D.

Yup, happy to share the dataset.
Both the image and the macro is in this folder.

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