From 4ca037593a6f57d718695ea89dde8855660e7f36 Mon Sep 17 00:00:00 2001 From: csmagnano Date: Wed, 10 Jan 2024 15:14:57 -0500 Subject: [PATCH] Initial pass on intro lesson, and moved all other lessons to narrow in on install issue --- episodes/intro_bioc_sce.Rmd | 68 +++++++++++++----- .../cell_type_annotation.Rmd | 0 {episodes => old-lessons}/eda_qc.Rmd | 0 ...RY_technical_cartoon_curatedAtlasQuery.png | Bin 0 -> 98614 bytes {episodes => old-lessons}/hca.Rmd | 0 {episodes => old-lessons}/large_data.Rmd | 0 {episodes => old-lessons}/multi_sample.Rmd | 0 7 files changed, 51 insertions(+), 17 deletions(-) rename {episodes => old-lessons}/cell_type_annotation.Rmd (100%) rename {episodes => old-lessons}/eda_qc.Rmd (100%) create mode 100644 old-lessons/figures/HCA_sccomp_SUPPLEMENTARY_technical_cartoon_curatedAtlasQuery.png rename {episodes => old-lessons}/hca.Rmd (100%) rename {episodes => old-lessons}/large_data.Rmd (100%) rename {episodes => old-lessons}/multi_sample.Rmd (100%) diff --git a/episodes/intro_bioc_sce.Rmd b/episodes/intro_bioc_sce.Rmd index e482f46..0e72bdc 100644 --- a/episodes/intro_bioc_sce.Rmd +++ b/episodes/intro_bioc_sce.Rmd @@ -1,14 +1,22 @@ --- title: Introduction to Bioconductor and the SingleCellExperiment class -vignette: > - % \VignetteIndexEntry{Introduction} - % \VignetteEngine{knitr::rmarkdown} - %\VignetteEncoding{UTF-8} -output: - html_document: - mathjax: null +teaching: 10 # Minutes of teaching in the lesson +exercises: 2 # Minutes of exercises in the lesson --- +:::::::::::::::::::::::::::::::::::::: questions + +- What is Bioconductor? +- How is single-cell data store in the Bioconductor ecosystem? + +:::::::::::::::::::::::::::::::::::::::::::::::: + +::::::::::::::::::::::::::::::::::::: objectives + +- TODO + +:::::::::::::::::::::::::::::::::::::::::::::::: + # Setup ```{r setup, message = FALSE, warning=FALSE} @@ -92,24 +100,50 @@ library(scater) plotReducedDim(sce, "pca.corrected.E8.5", colour_by = "celltype.mapped") ``` +:::::::::::::::::::::::::::::::::: challenge -# Session Info +#### Exercise 1 -```{r sessionInfo} -sessionInfo() -``` +Create a `SingleCellExperiment` object: Try and create a SingleCellExperiment object "from scratch". Start from a matrix (either randomly generated or with some fake data in it) and add one or more columns as colData. -# Further Reading +:::::::::::::: hint -* OSCA book, [Introduction](https://bioconductor.org/books/release/OSCA.intro) +The `SingleCellExperiment` function can be used to create a new SingleCellExperiment object + +::::::::::::::::::::::: + +:::::::::::::: solution + +TODO +::::::::::::::::::::::: + +::::::::::::::::::::::::::::::::::::::::::::: -# Exercises +:::::::::::::::::::::::::::::::::: challenge -1. Create a `SingleCellExperiment` object: Try and create a SingleCellExperiment object "from scratch". Start from a matrix (either randomly generated or with some fake data in it) and add one or more columns as colData. +#### Exercise 2 + +Combining two objects: The `MouseGastrulationData` package contains several datasets. Download sample 6 of the chimera experiment by running `sce6 <- WTChimeraData(sample=6)`. Use the `cbind` function to combine the new data with the `sce` object created before. + +:::::::::::::: solution + +TODO +::::::::::::::::::::::: + +::::::::::::::::::::::::::::::::::::::::::::: + + +::::::::::::::::::::::::::::::::::::: keypoints + +- TODO + +:::::::::::::::::::::::::::::::::::::::::::::::: + +# Further Reading + +* OSCA book, [Introduction](https://bioconductor.org/books/release/OSCA.intro) -Hint: the `SingleCellExperiment` function can be used to create a new SingleCellExperiment object. -2. Combining two objects: The `MouseGastrulationData` package contains several datasets. Download sample 6 of the chimera experiment by running `sce6 <- WTChimeraData(sample=6)`. 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