diff --git a/README.md b/README.md index b3f0a9c..a372e94 100644 --- a/README.md +++ b/README.md @@ -2,10 +2,25 @@ # bigdataviewer-omezarr +Building and Installation +------------------------- + +`bigdataviewer-omezarr` releases are available via the `AllenNeuralDynamics` (unlisted) Fiji update site. +In Fiji, go to Update... -> Advanced mode -> Manage Update Sites -> Add Unlisted Site. Enter name `AllenNeuralDynamics` and URL `https://sites.imagej.net/AllenNeuralDynamics/`. After refreshing, `jars/bigdataviewer-omezarr.jar` will be available for installation. + +For more information on Fiji update sites, please visit the [imagej documentation](https://imagej.net/update-sites/setup). + +Alternatively, the git repository checkout can be built by maven: +```shell +mvn clean install +``` +then copy the compiled snapshot version jar (e.g. `bigdataviewer-omezarr-0.2.2-SNAPSHOT.jar`) +from the local `target/` folder into Fiji's `jars/` folder. + OME-Zarr support ---------------- -This package provides OME-Zarr reading support to bigdataviewer. In addition to the OME-NGFF json metadata, a +This package provides OME-Zarr reading support to bigdataviewer and BigStitcher. In addition to the OME-NGFF json metadata, a bigdataviewer `dataset.xml` dataset definition is required that refers to the image format `bdv.multimg.zarr`. Currently, two basic loader classes are provided: `XmlIoZarrImageLoader` and `ZarrImageLoader`. @@ -23,20 +38,25 @@ The OME-NGFF layouts supported by this package is limited. Most notably: * `unit` in `axes` definitions in `.zattrs` are ignored. The same units are implicitly assumed across images. Use `voxelSize` in `dataset.xml` to define physical units. -* For multiresolution images, the first dataset defined in the `.zattrs` must be the raw (finest) resolution. Anisotropy +* For multi-resolution images, the first dataset defined in the `.zattrs` must be the raw (finest) resolution. Anisotropy defined for the raw resolution in `.zattrs` are ignored, use `voxelSize` in `dataset.xml` instead. Downsampling factors - are determined from `coordinateTransformations` compared to the raw resolution. + are determined from `coordinateTransformations` compared to the raw resolution. Only factor of 2 downsampling sequences + have been tested. -* Multiple images must be located in separate zgroup folders. Only one image per top level `.zattrs` file allowed +* Multiple images must be located in separate zgroup folders. Only one image per top level `.zattrs` file is allowed (multiple entries in the `multiscales` section are disregarded). bdv.multimg.zarr format ----------------------- -The `dataset.xml` file should define the location of the zarr image for each _view_ in the following format: +The `dataset.xml` file should define the location of the zarr image for each _view_ in the following format. + +### Local filesystem access + ```xml + /absolute_path_to_zarr_root @@ -47,13 +67,35 @@ The `dataset.xml` file should define the location of the zarr image for each _vi ``` -Build instructions ------------------- +### Direct S3 access -Build and install: +Direct AWS S3 access is also supported. It is triggered by the presence of the `` tag in the xml: -```shell -mvn clean install +```xml + + aind-open-data + + prefix_to_zarr_root_within_s3_bucket + + + relative_path_from_zarr_root + + ... + + +``` + +Note: The xml file itself still must be a local filesystem file. + +S3 credentials must be accessible to the default AWS authentication chain. In +most cases, credentials may be set in environment variables. While there is a fallback to try anonymous access if none +is provided, authentication is usually faster even to open data buckets with explicit credentials. + +Also, `AWS_REGION` and `AWS_DEFAULT_REGION` must be set. + +``` +export AWS_REGION=us-west-2 +export AWS_DEFAULT_REGION=us-west-2 ``` Acknowledgement diff --git a/src/main/java/bdv/img/omezarr/MultiscaleImage.java b/src/main/java/bdv/img/omezarr/MultiscaleImage.java index 9cfdf3c..d44e5cd 100644 --- a/src/main/java/bdv/img/omezarr/MultiscaleImage.java +++ b/src/main/java/bdv/img/omezarr/MultiscaleImage.java @@ -202,6 +202,13 @@ public ZarrKeyValueReader build() throws N5Exception { return new N5ZarrReader(multiscalePath); } } + /** + * Getter for s3 mode. + * @return True if S3 mode is active. + */ + public boolean isS3Mode() { + return s3Mode; + } /** * New reader builder for a sub-image. diff --git a/src/main/java/bdv/img/omezarr/XmlIoZarrImageLoader.java b/src/main/java/bdv/img/omezarr/XmlIoZarrImageLoader.java index 5cd09b7..286ed71 100644 --- a/src/main/java/bdv/img/omezarr/XmlIoZarrImageLoader.java +++ b/src/main/java/bdv/img/omezarr/XmlIoZarrImageLoader.java @@ -6,13 +6,13 @@ * %% * Redistribution and use in source and binary forms, with or without * modification, are permitted provided that the following conditions are met: - * + * * 1. Redistributions of source code must retain the above copyright notice, * this list of conditions and the following disclaimer. * 2. Redistributions in binary form must reproduce the above copyright notice, * this list of conditions and the following disclaimer in the documentation * and/or other materials provided with the distribution. - * + * * THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" * AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE * IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE @@ -33,15 +33,11 @@ import bdv.ViewerSetupImgLoader; import bdv.spimdata.SpimDataMinimal; import bdv.spimdata.XmlIoSpimDataMinimal; -import com.amazonaws.auth.AWSCredentials; -import com.amazonaws.auth.AWSStaticCredentialsProvider; -import com.amazonaws.auth.AnonymousAWSCredentials; -import com.amazonaws.auth.DefaultAWSCredentialsProviderChain; -import com.amazonaws.services.s3.AmazonS3; -import com.amazonaws.services.s3.AmazonS3ClientBuilder; + import java.io.File; import java.util.Map; import java.util.TreeMap; + import mpicbg.spim.data.SpimDataException; import mpicbg.spim.data.XmlHelpers; import mpicbg.spim.data.generic.sequence.AbstractSequenceDescription; @@ -53,27 +49,28 @@ import static mpicbg.spim.data.XmlHelpers.loadPath; import static mpicbg.spim.data.XmlKeys.IMGLOADER_FORMAT_ATTRIBUTE_NAME; -@ImgLoaderIo( format = "bdv.multimg.zarr", type = ZarrImageLoader.class ) -public class XmlIoZarrImageLoader implements XmlIoBasicImgLoader -{ +@ImgLoaderIo(format = "bdv.multimg.zarr", type = ZarrImageLoader.class) +public class XmlIoZarrImageLoader implements XmlIoBasicImgLoader { @Override - public Element toXml(final ZarrImageLoader imgLoader, final File basePath ) - { - final Element e_imgloader = new Element( "ImageLoader" ); + public Element toXml(final ZarrImageLoader imgLoader, final File basePath) { + final Element e_imgloader = new Element("ImageLoader"); final MultiscaleImage.ZarrKeyValueReaderBuilder readerBuilder = imgLoader.getZarrKeyValueReaderBuilder(); - e_imgloader.setAttribute( IMGLOADER_FORMAT_ATTRIBUTE_NAME, "bdv.multimg.zarr" ); - e_imgloader.setAttribute( "version", "1.0" ); - e_imgloader.addContent(XmlHelpers.pathElement("zarr", - new File(readerBuilder.getMultiscalePath()), null)); - if (readerBuilder.getBucketName()!=null) - { + e_imgloader.setAttribute(IMGLOADER_FORMAT_ATTRIBUTE_NAME, "bdv.multimg.zarr"); + e_imgloader.setAttribute("version", "1.0"); + if (readerBuilder.isS3Mode()) { final Element e_bucket = new Element("s3bucket"); e_bucket.addContent(readerBuilder.getBucketName()); e_imgloader.addContent(e_bucket); + final Element e_zarr = new Element("zarr"); + e_zarr.setAttribute("type", "absolute"); + e_zarr.addContent(readerBuilder.getMultiscalePath()); + e_imgloader.addContent(e_zarr); + } else { + e_imgloader.addContent(XmlHelpers.pathElement("zarr", + new File(readerBuilder.getMultiscalePath()), null)); } final Element e_zgroups = new Element("zgroups"); - for (final Map.Entry ze: imgLoader.getZgroups().entrySet()) - { + for (final Map.Entry ze : imgLoader.getZgroups().entrySet()) { final ViewId vId = ze.getKey(); final Element e_zgroup = new Element("zgroup"); e_zgroup.setAttribute("setup", String.valueOf(vId.getViewSetupId())); @@ -88,27 +85,23 @@ public Element toXml(final ZarrImageLoader imgLoader, final File basePath ) } @Override - public ZarrImageLoader fromXml(final Element elem, final File basePath, final AbstractSequenceDescription< ?, ?, ? > sequenceDescription ) - { - final Element zgroupsElem = elem.getChild( "zgroups" ); - final TreeMap zgroups = new TreeMap<>(); + public ZarrImageLoader fromXml(final Element elem, final File basePath, + final AbstractSequenceDescription sequenceDescription) { + final Element zgroupsElem = elem.getChild("zgroups"); + final TreeMap zgroups = new TreeMap<>(); // TODO validate that sequenceDescription and zgroups have the same entries - for ( final Element c : zgroupsElem.getChildren( "zgroup" ) ) - { - final int timepointId = Integer.parseInt( c.getAttributeValue( "timepoint" ) ); - final int setupId = Integer.parseInt( c.getAttributeValue( "setup" ) ); - final String path = c.getChild( "path" ).getText(); - zgroups.put( new ViewId( timepointId,setupId ), path ); + for (final Element c : zgroupsElem.getChildren("zgroup")) { + final int timepointId = Integer.parseInt(c.getAttributeValue("timepoint")); + final int setupId = Integer.parseInt(c.getAttributeValue("setup")); + final String path = c.getChild("path").getText(); + zgroups.put(new ViewId(timepointId, setupId), path); } final Element s3Bucket = elem.getChild("s3bucket"); final MultiscaleImage.ZarrKeyValueReaderBuilder keyValueReaderBuilder; - if (s3Bucket==null) - { - final File zpath = loadPath( elem, "zarr", basePath ); + if (s3Bucket == null) { + final File zpath = loadPath(elem, "zarr", basePath); keyValueReaderBuilder = new MultiscaleImage.ZarrKeyValueReaderBuilder(zpath.getAbsolutePath()); - } - else - { + } else { // `File` class should not be used for uri manipulation as it replaces slashes with backslashes on Windows keyValueReaderBuilder = new MultiscaleImage.ZarrKeyValueReaderBuilder(s3Bucket.getText(), elem.getChildText("zarr")); @@ -116,20 +109,19 @@ public ZarrImageLoader fromXml(final Element elem, final File basePath, final Ab return new ZarrImageLoader(keyValueReaderBuilder, zgroups, sequenceDescription); } - public static void main( String[] args ) throws SpimDataException - { + public static void main(String[] args) throws SpimDataException { // final String fn = "/home/gkovacs/data/davidf_zarr_dataset.xml"; final String fn = "/home/gabor.kovacs/data/davidf_zarr_dataset.xml"; // final String fn = "/home/gabor.kovacs/data/zarr_reader_test_2022-11-16/bdv_zarr_test3.xml"; // final String fn = "/Users/kgabor/data/davidf_zarr_dataset.xml"; - final SpimDataMinimal spimData = new XmlIoSpimDataMinimal().load( fn ); - final ViewerImgLoader imgLoader = ( ViewerImgLoader ) spimData.getSequenceDescription().getImgLoader(); + final SpimDataMinimal spimData = new XmlIoSpimDataMinimal().load(fn); + final ViewerImgLoader imgLoader = (ViewerImgLoader) spimData.getSequenceDescription().getImgLoader(); final ViewerSetupImgLoader setupImgLoader = imgLoader.getSetupImgLoader(0); int d = setupImgLoader.getImage(0).numDimensions(); setupImgLoader.getMipmapResolutions(); // BigDataViewer.open(spimData, "BigDataViewer Zarr Example", new ProgressWriterConsole(), ViewerOptions.options()); - System.out.println( "imgLoader = " + imgLoader ); - System.out.println( "setupimgLoader = " + setupImgLoader ); + System.out.println("imgLoader = " + imgLoader); + System.out.println("setupimgLoader = " + setupImgLoader); } }