diff --git a/sample-apps/python-auto-instrumentation-sample-app/requirements.txt b/sample-apps/python-auto-instrumentation-sample-app/requirements.txt
index 8d5f753d..95e740b1 100644
--- a/sample-apps/python-auto-instrumentation-sample-app/requirements.txt
+++ b/sample-apps/python-auto-instrumentation-sample-app/requirements.txt
@@ -1,11 +1,11 @@
-boto3==1.33.12
+boto3==1.34.7
 Flask==2.3.3
-opentelemetry-distro==0.42b0
-opentelemetry-exporter-otlp==1.21.0
-opentelemetry-api==1.21.0
-opentelemetry-instrumentation-flask==0.42b0
-opentelemetry-instrumentation-requests==0.42b0
-opentelemetry-instrumentation-botocore==0.42b0
+opentelemetry-distro==0.43b0
+opentelemetry-exporter-otlp==1.22.0
+opentelemetry-api==1.22.0
+opentelemetry-instrumentation-flask==0.43b0
+opentelemetry-instrumentation-requests==0.43b0
+opentelemetry-instrumentation-botocore==0.43b0
 opentelemetry-sdk-extension-aws==2.0.1
 opentelemetry-propagator-aws-xray==1.0.1
 protobuf==4.25.1