diff --git a/sample-apps/python-manual-instrumentation-sample-app/requirements.txt b/sample-apps/python-manual-instrumentation-sample-app/requirements.txt
index 71cd965f..24a85e77 100644
--- a/sample-apps/python-manual-instrumentation-sample-app/requirements.txt
+++ b/sample-apps/python-manual-instrumentation-sample-app/requirements.txt
@@ -1,13 +1,13 @@
-boto3==1.35.64
+boto3==1.35.87
 Flask==2.3.3
-opentelemetry-distro==0.49b2
-opentelemetry-exporter-otlp==1.28.2
-opentelemetry-api==1.28.2
-opentelemetry-instrumentation-flask==0.49b2
-opentelemetry-instrumentation-requests==0.49b2
-opentelemetry-instrumentation-botocore==0.49b2
-opentelemetry-sdk-extension-aws==2.0.2
+opentelemetry-distro==0.50b0
+opentelemetry-exporter-otlp==1.29.0
+opentelemetry-api==1.29.0
+opentelemetry-instrumentation-flask==0.50b0
+opentelemetry-instrumentation-requests==0.50b0
+opentelemetry-instrumentation-botocore==0.50b0
+opentelemetry-sdk-extension-aws==2.1.0
 opentelemetry-propagator-aws-xray==1.0.2
-protobuf==5.28.3
+protobuf==5.29.2
 PyYAML==6.0.2
 requests==2.32.3
\ No newline at end of file