diff --git a/includes/Checker/Checks/Plugin_Repo/Plugin_Readme_Check.php b/includes/Checker/Checks/Plugin_Repo/Plugin_Readme_Check.php
index ba9c7b859..ab949b49f 100644
--- a/includes/Checker/Checks/Plugin_Repo/Plugin_Readme_Check.php
+++ b/includes/Checker/Checks/Plugin_Repo/Plugin_Readme_Check.php
@@ -104,6 +104,9 @@ protected function check_files( Check_Result $result, array $files ) {
 		// Check the readme file for warnings.
 		$this->check_for_warnings( $result, $readme_file, $parser );
 
+		// Check the readme file for donate link.
+		$this->check_for_donate_link( $result, $readme_file, $parser );
+
 		// Check the readme file for contributors.
 		$this->check_for_contributors( $result, $readme_file );
 	}
@@ -601,6 +604,41 @@ private function check_for_warnings( Check_Result $result, string $readme_file,
 		}
 	}
 
+	/**
+	 * Checks the readme file for donate link.
+	 *
+	 * @since 1.3.0
+	 *
+	 * @param Check_Result $result      The Check Result to amend.
+	 * @param string       $readme_file Readme file.
+	 * @param Parser       $parser      The Parser object.
+	 */
+	private function check_for_donate_link( Check_Result $result, string $readme_file, Parser $parser ) {
+		$donate_link = $parser->donate_link;
+
+		// Bail if empty donate link.
+		if ( empty( $donate_link ) ) {
+			return;
+		}
+
+		if ( ! ( filter_var( $donate_link, FILTER_VALIDATE_URL ) === $donate_link && str_starts_with( $donate_link, 'http' ) ) ) {
+			$this->add_result_warning_for_file(
+				$result,
+				sprintf(
+					/* translators: %s: plugin header field */
+					__( 'The "%s" header in the readme file must be a valid URL.', 'plugin-check' ),
+					'Donate link'
+				),
+				'readme_invalid_donate_link',
+				$readme_file,
+				0,
+				0,
+				'https://developer.wordpress.org/plugins/wordpress-org/how-your-readme-txt-works/#readme-header-information',
+				6
+			);
+		}
+	}
+
 	/**
 	 * Checks the readme file for contributors.
 	 *
diff --git a/tests/phpunit/testdata/plugins/test-plugin-plugin-readme-md-with-errors/readme.md b/tests/phpunit/testdata/plugins/test-plugin-plugin-readme-md-with-errors/readme.md
index a6e6ce7e9..68637e562 100644
--- a/tests/phpunit/testdata/plugins/test-plugin-plugin-readme-md-with-errors/readme.md
+++ b/tests/phpunit/testdata/plugins/test-plugin-plugin-readme-md-with-errors/readme.md
@@ -9,5 +9,6 @@ Stable tag:        trunk
 License:           Oculus VR Inc. Software Development Kit License
 License URI:       https://www.gnu.org/licenses/gpl-2.0.html
 Tags:              performance, testing, security
+Donate link:       not-a-valid-url
 
 Here is a short description of the plugin.
diff --git a/tests/phpunit/tests/Checker/Checks/Plugin_Readme_Check_Tests.php b/tests/phpunit/tests/Checker/Checks/Plugin_Readme_Check_Tests.php
index 275da69c0..a478c22f6 100644
--- a/tests/phpunit/tests/Checker/Checks/Plugin_Readme_Check_Tests.php
+++ b/tests/phpunit/tests/Checker/Checks/Plugin_Readme_Check_Tests.php
@@ -234,6 +234,7 @@ public function test_run_md_with_errors() {
 
 		$this->assertCount( 1, wp_list_filter( $warnings['readme.md'][0][0], array( 'code' => 'mismatched_plugin_name' ) ) );
 		$this->assertCount( 1, wp_list_filter( $warnings['readme.md'][0][0], array( 'code' => 'readme_invalid_contributors' ) ) );
+		$this->assertCount( 1, wp_list_filter( $warnings['readme.md'][0][0], array( 'code' => 'readme_invalid_donate_link' ) ) );
 	}
 
 	public function test_single_file_plugin_without_error_for_trademarks() {