Reading in 8-bit images

[nilseling]

Hi all,

I just stumbled upon this by chance. Looking at the mean pixel intensity of CK across all cells it seemed to me that these intensities are quite low. So I checked and you should be using the as.is = TRUE option in the cytomapper::loadImages function when reading in 8-bit images. I always tend to compare the pixel intensities of the CytoImageList object against the ones I get from ImageJ/FIJI and there you can see that not setting as.is = TRUE divides the true pixel intensities by 2^8 - 1 = 255. It's a bit of an annoying thing and comes from how tiff::readTIFF processes integers when reading in images.