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Off by one error in DoG detection? #122

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lacan opened this issue Mar 3, 2023 · 2 comments
Open

Off by one error in DoG detection? #122

lacan opened this issue Mar 3, 2023 · 2 comments

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@lacan
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lacan commented Mar 3, 2023

Tried to perform a DoG on a large amount of DAPI tiles that have some 3D to them. Some Z are empty and not others, which was leading to a strange behaviour that makes me think that the Z slice index is off by one.

to reproduce and see
Import a bunch of tiles, make sure that they do not start or end at the same Z slice, this helps to view
Alternatively, I guess a couple stacks with very different points-like objects in Z would work. Then you would think that the LoG detector is not working, as the detected rois won't overlap with the point objects on the image.

Here is a small GIF

offbyonebigstitcher

I am using the latest version as per the Fiji Update Site of BigStitcher.

@lacan
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lacan commented Mar 6, 2023

Hello,
Should I provide further information?

@StephanPreibisch
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Hi, could you please send me the image? I didn't find an obvious bug ...

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