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Tried to perform a DoG on a large amount of DAPI tiles that have some 3D to them. Some Z are empty and not others, which was leading to a strange behaviour that makes me think that the Z slice index is off by one.
to reproduce and see
Import a bunch of tiles, make sure that they do not start or end at the same Z slice, this helps to view
Alternatively, I guess a couple stacks with very different points-like objects in Z would work. Then you would think that the LoG detector is not working, as the detected rois won't overlap with the point objects on the image.
Here is a small GIF
I am using the latest version as per the Fiji Update Site of BigStitcher.
The text was updated successfully, but these errors were encountered:
Tried to perform a DoG on a large amount of DAPI tiles that have some 3D to them. Some Z are empty and not others, which was leading to a strange behaviour that makes me think that the Z slice index is off by one.
to reproduce and see
Import a bunch of tiles, make sure that they do not start or end at the same Z slice, this helps to view
Alternatively, I guess a couple stacks with very different points-like objects in Z would work. Then you would think that the LoG detector is not working, as the detected rois won't overlap with the point objects on the image.
Here is a small GIF
I am using the latest version as per the Fiji Update Site of BigStitcher.
The text was updated successfully, but these errors were encountered: