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Error running Prodigal on genome #566

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2 tasks
fhdbqr opened this issue Dec 20, 2023 · 4 comments
Open
2 tasks

Error running Prodigal on genome #566

fhdbqr opened this issue Dec 20, 2023 · 4 comments
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error Help required for a GTDB-Tk error.

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@fhdbqr
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fhdbqr commented Dec 20, 2023

Environment

  • Installed via pip (include the output of pip list)
  • [ √] Using a conda environment (include the output of conda list && conda list --revisions)
  • Using a Docker container (include the IMAGE ID of the container)

Server information

  • CPU: Intel(R) Xeon(R) CPU E5-4657L v2 @ 2.40GHz 48
  • RAM: 792598980 kB
  • OS: Ubuntu 20.04.4 LTS

Debugging information

  • [√] gtdbtk.log has been included (drag and drop the file to upload).
  • [] Genomes have been included (if possible, and there are few).

Additional comments

Hello everyone, I've come across an error while running 'classify_wf' or 'identify' modules': 'Error running Prodigal on the genome, free(): invalid pointer. This genome is skipped.' However, there were no details in 'gtdbtk.failed_genomes.tsv'.
Interestingly, when I individually run Prodigal V2.6.3 with these bins, no errors arise. Any suggestions on how to resolve this?
gtdbtk.log
gtdbtk.failed_genomes.txt

@fhdbqr fhdbqr added the error Help required for a GTDB-Tk error. label Dec 20, 2023
@pchaumeil
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Hello,
Apologies for the very late reply.
Do you still have an issue with Prodigal?

@fhdbqr
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fhdbqr commented Apr 16, 2024

Hello,
Yes, this error occurred during the execution of certain MAGs; however, the overall process was successfully completed.

@pchaumeil
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Could you please send few MAGS generating this error?
Thanks,
Pierre

@fhdbqr
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fhdbqr commented Apr 22, 2024

I'm sorry for the delay. Here's an example where the aforementioned problem might occur. It contains a 'binning' file and an output folder from GTDBTk.
example.tar.gz

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Labels
error Help required for a GTDB-Tk error.
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