diff --git a/include/highfive/bits/H5Slice_traits.hpp b/include/highfive/bits/H5Slice_traits.hpp
index 4d40b7797..65c0d39e0 100644
--- a/include/highfive/bits/H5Slice_traits.hpp
+++ b/include/highfive/bits/H5Slice_traits.hpp
@@ -13,6 +13,7 @@
 
 #include "H5_definitions.hpp"
 #include "H5Utils.hpp"
+#include "convert_size_vector.hpp"
 
 #include "../H5PropertyList.hpp"
 #include "h5s_wrapper.hpp"
@@ -51,17 +52,6 @@ class ElementSet {
     friend class SliceTraits;
 };
 
-namespace detail {
-
-template <class To, class From>
-inline std::vector<To> convertSizeVector(const std::vector<From>& from) {
-    std::vector<To> to(from.size());
-    std::copy(from.cbegin(), from.cend(), to.begin());
-
-    return to;
-}
-}  // namespace detail
-
 inline std::vector<hsize_t> toHDF5SizeVector(const std::vector<size_t>& from) {
     return detail::convertSizeVector<hsize_t>(from);
 }
diff --git a/include/highfive/bits/convert_size_vector.hpp b/include/highfive/bits/convert_size_vector.hpp
new file mode 100644
index 000000000..62a815ae5
--- /dev/null
+++ b/include/highfive/bits/convert_size_vector.hpp
@@ -0,0 +1,31 @@
+/*
+ *  Copyright (c), 2017, Adrien Devresse <adrien.devresse@epfl.ch>
+ *  Copyright (c), 2017-2024, BlueBrain Project, EPFL
+ *
+ *  Distributed under the Boost Software License, Version 1.0.
+ *    (See accompanying file LICENSE_1_0.txt or copy at
+ *          http://www.boost.org/LICENSE_1_0.txt)
+ *
+ */
+#pragma once
+
+#include <vector>
+
+namespace HighFive {
+namespace detail {
+
+template <class To, class From, class It = From const*>
+inline std::vector<To> convertSizeVector(const It& begin, const It& end) {
+    std::vector<To> to(static_cast<size_t>(end - begin));
+    std::copy(begin, end, to.begin());
+
+    return to;
+}
+
+template <class To, class From>
+inline std::vector<To> convertSizeVector(const std::vector<From>& from) {
+    return convertSizeVector<To, From>(from.cbegin(), from.cend());
+}
+
+}  // namespace detail
+}  // namespace HighFive
diff --git a/include/highfive/experimental/opencv.hpp b/include/highfive/experimental/opencv.hpp
new file mode 100644
index 000000000..224160975
--- /dev/null
+++ b/include/highfive/experimental/opencv.hpp
@@ -0,0 +1,149 @@
+#pragma once
+
+#include "../bits/H5Inspector_decl.hpp"
+#include "../H5Exception.hpp"
+
+#include <opencv2/opencv.hpp>
+
+#include "../bits/convert_size_vector.hpp"
+
+namespace HighFive {
+namespace details {
+
+
+template <class T>
+struct inspector<cv::Mat_<T>> {
+    using type = cv::Mat_<T>;
+    using value_type = T;
+    using base_type = typename inspector<value_type>::base_type;
+    using hdf5_type = base_type;
+
+    static void assert_row_major(const type& type) {
+        // Documentation claims that Mat_ is always row-major. However, it
+        // could be padded. The steps/strides are in bytes.
+        int rank = type.dims;
+        size_t ld = sizeof(T);
+        for (int i = rank - 1; i >= 0; --i) {
+            if (static_cast<size_t>(type.step[i]) != ld) {
+                throw DataSetException("Padded cv::Mat_ are not supported.");
+            }
+
+            ld *= static_cast<size_t>(type.size[i]);
+        }
+    }
+
+
+    static constexpr size_t min_ndim = 2 + inspector<value_type>::min_ndim;
+    static constexpr size_t max_ndim = 1024 + inspector<value_type>::max_ndim;
+
+    // HighFive doesn't support padded OpenCV arrays. Therefore, pretend
+    // that they themselves are trivially copyable. And error out if the
+    // assumption is violated.
+    static constexpr bool is_trivially_copyable = std::is_trivially_copyable<value_type>::value &&
+                                                  inspector<value_type>::is_trivially_nestable;
+    static constexpr bool is_trivially_nestable = false;
+
+    static size_t getRank(const type& val) {
+        if (val.empty()) {
+            return min_ndim;
+
+        } else {
+            return static_cast<size_t>(val.dims) +
+                   inspector<value_type>::getRank(getAnyElement(val));
+        }
+    }
+
+    static const T& getAnyElement(const type& val) {
+        return *reinterpret_cast<T const*>(val.data);
+    }
+
+    static T& getAnyElement(type& val) {
+        return *reinterpret_cast<T*>(val.data);
+    }
+
+    static size_t getLocalRank(const type& val) {
+        return static_cast<size_t>(val.dims);
+    }
+
+    static std::vector<size_t> getDimensions(const type& val) {
+        auto local_rank = getLocalRank(val);
+        auto rank = getRank(val);
+        std::vector<size_t> dims(rank, 1ul);
+
+        if (val.empty()) {
+            dims[0] = 0ul;
+            dims[1] = 1ul;
+            return dims;
+        }
+
+        for (size_t i = 0; i < local_rank; ++i) {
+            dims[i] = static_cast<size_t>(val.size[static_cast<int>(i)]);
+        }
+
+        auto s = inspector<value_type>::getDimensions(getAnyElement(val));
+        std::copy(s.cbegin(), s.cend(), dims.begin() + static_cast<int>(local_rank));
+        return dims;
+    }
+
+    static void prepare(type& val, const std::vector<size_t>& dims) {
+        auto subdims = detail::convertSizeVector<int>(dims);
+        val.create(static_cast<int>(subdims.size()), subdims.data());
+    }
+
+    static hdf5_type* data(type& val) {
+        assert_row_major(val);
+
+        if (!is_trivially_copyable) {
+            throw DataSetException("Invalid used of `inspector<Eigen::Matrix<...>>::data`.");
+        }
+
+        if (val.empty()) {
+            return nullptr;
+        }
+
+        return inspector<value_type>::data(getAnyElement(val));
+    }
+
+    static const hdf5_type* data(const type& val) {
+        assert_row_major(val);
+
+        if (!is_trivially_copyable) {
+            throw DataSetException("Invalid used of `inspector<Eigen::Matrix<...>>::data`.");
+        }
+
+        if (val.empty()) {
+            return nullptr;
+        }
+
+        return inspector<value_type>::data(getAnyElement(val));
+    }
+
+    static void serialize(const type& val, const std::vector<size_t>& dims, hdf5_type* m) {
+        if (val.empty()) {
+            return;
+        }
+
+        auto local_rank = val.dims;
+        auto subdims = std::vector<size_t>(dims.begin() + local_rank, dims.end());
+        auto subsize = compute_total_size(subdims);
+        for (auto it = val.begin(); it != val.end(); ++it) {
+            inspector<value_type>::serialize(*it, subdims, m);
+            m += subsize;
+        }
+    }
+
+    static void unserialize(const hdf5_type* vec_align,
+                            const std::vector<size_t>& dims,
+                            type& val) {
+        auto local_rank = val.dims;
+        auto subdims = std::vector<size_t>(dims.begin() + local_rank, dims.end());
+        auto subsize = compute_total_size(subdims);
+        for (auto it = val.begin(); it != val.end(); ++it) {
+            inspector<value_type>::unserialize(vec_align, subdims, *it);
+            vec_align += subsize;
+        }
+    }
+};
+
+}  // namespace details
+}  // namespace HighFive
diff --git a/tests/unit/CMakeLists.txt b/tests/unit/CMakeLists.txt
index a282da319..0f795812d 100644
--- a/tests/unit/CMakeLists.txt
+++ b/tests/unit/CMakeLists.txt
@@ -6,7 +6,7 @@ if(MSVC)
 endif()
 
 ## Base tests
-foreach(test_name tests_high_five_base tests_high_five_multi_dims tests_high_five_easy test_all_types test_high_five_selection tests_high_five_data_type test_empty_arrays test_legacy test_string)
+foreach(test_name tests_high_five_base tests_high_five_multi_dims tests_high_five_easy test_all_types test_high_five_selection tests_high_five_data_type test_empty_arrays test_legacy test_opencv test_string)
   add_executable(${test_name} "${test_name}.cpp")
   target_link_libraries(${test_name} HighFive HighFiveWarnings HighFiveFlags Catch2::Catch2WithMain)
   target_link_libraries(${test_name} HighFiveOptionalDependencies)
@@ -63,6 +63,10 @@ foreach(PUBLIC_HEADER ${public_headers})
       continue()
     endif()
 
+    if(PUBLIC_HEADER STREQUAL "highfive/opencv.hpp" AND NOT HIGHFIVE_TEST_OPENCV)
+      continue()
+    endif()
+
     get_filename_component(CLASS_NAME ${PUBLIC_HEADER} NAME_WE)
     configure_file(tests_import_public_headers.cpp "tests_${CLASS_NAME}.cpp" @ONLY)
     add_executable("tests_include_${CLASS_NAME}" "${CMAKE_CURRENT_BINARY_DIR}/tests_${CLASS_NAME}.cpp")
diff --git a/tests/unit/test_opencv.cpp b/tests/unit/test_opencv.cpp
new file mode 100644
index 000000000..b27c06daa
--- /dev/null
+++ b/tests/unit/test_opencv.cpp
@@ -0,0 +1,59 @@
+/*
+ *  Copyright (c), 2024, Blue Brain Project - EPFL
+ *
+ *  Distributed under the Boost Software License, Version 1.0.
+ *    (See accompanying file LICENSE_1_0.txt or copy at
+ *          http://www.boost.org/LICENSE_1_0.txt)
+ *
+ */
+
+#if HIGHFIVE_TEST_OPENCV
+
+#include <catch2/catch_test_macros.hpp>
+#include <catch2/catch_template_test_macros.hpp>
+#include <catch2/matchers/catch_matchers_vector.hpp>
+
+#include <highfive/highfive.hpp>
+#include <highfive/experimental/opencv.hpp>
+#include "tests_high_five.hpp"
+#include "create_traits.hpp"
+
+using namespace HighFive;
+using Catch::Matchers::Equals;
+
+TEST_CASE("OpenCV") {
+    auto file = File("rw_opencv.h5", File::Truncate);
+
+    auto a = cv::Mat_<double>(3, 5);
+    auto dset = file.createDataSet("a", a);
+    auto b = dset.read<cv::Mat_<double>>();
+    REQUIRE(a(0, 0) == b(0, 0));
+
+    auto va = std::vector<cv::Mat_<double>>(7, cv::Mat_<double>(3, 5));
+    auto vdset = file.createDataSet("va", va);
+    auto vb = vdset.read<std::vector<cv::Mat_<double>>>();
+    REQUIRE(vb.size() == va.size());
+    REQUIRE(vb[0](0, 0) == va[0](0, 0));
+}
+
+TEST_CASE("OpenCV subarrays") {
+    auto file = File("rw_opencv_subarray.h5", File::Truncate);
+
+    auto a = cv::Mat_<double>(3, 13);
+
+    SECTION("write") {
+        auto sa = cv::Mat_<double>(a.colRange(1, 4));
+        REQUIRE_THROWS(file.createDataSet("a", sa));
+    }
+
+    SECTION("read") {
+        auto b = cv::Mat_<double>(3, 17);
+        auto sb = cv::Mat_<double>(a.colRange(0, 13));
+        auto dset = file.createDataSet("a", a);
+
+        // Creates a new `Mat_` in `sb`.
+        dset.read(sb);
+    }
+}
+
+#endif