diff --git a/.github/workflows/daily-rocker-builds.yaml b/.github/workflows/daily-rocker-builds.yaml
index 7f98566..670af84 100644
--- a/.github/workflows/daily-rocker-builds.yaml
+++ b/.github/workflows/daily-rocker-builds.yaml
@@ -6,7 +6,7 @@ on:
       rver:
         default: "devel"
       biocver:
-        default: "3.20"
+        default: "3.21"
       check-rocker-image:
         default: "rocker/rstudio"
   schedule:
@@ -21,7 +21,7 @@ jobs:
       id: defs
       run: |
         echo rver=$(echo ${{ github.event.inputs.rver || 'devel' }}) >> $GITHUB_OUTPUT
-        BIOCVER=$(echo ${{ github.event.inputs.biocver || '3.20' }})
+        BIOCVER=$(echo ${{ github.event.inputs.biocver || '3.21' }})
         echo "develbiocver=$BIOCVER" >> $GITHUB_OUTPUT
         echo check-rocker-image=$(echo ${{ github.event.inputs.check-rocker-image || 'rocker/rstudio' }}) >> $GITHUB_OUTPUT
         RELEASE_BIOC_VER=$(echo "${BIOCVER%.*}.$((${BIOCVER##*.}-1))")
@@ -87,7 +87,7 @@ jobs:
       id: defs
       run: |
         echo rver=$(echo ${{ github.event.inputs.rver || 'devel' }}) >> $GITHUB_OUTPUT
-        echo biocver=$(echo ${{ github.event.inputs.biocver || '3.20' }}) >> $GITHUB_OUTPUT
+        echo biocver=$(echo ${{ github.event.inputs.biocver || '3.21' }}) >> $GITHUB_OUTPUT
         echo registryuser=$(echo ${{ github.repository_owner }} | awk '{print tolower($0)}') >> $GITHUB_OUTPUT
         echo rockerintermediateprefix=$(echo "ghcr.io/${{ github.repository_owner }}/rocker" | awk '{print tolower($0)}') >> $GITHUB_OUTPUT
 
@@ -139,7 +139,7 @@ jobs:
       id: defs
       run: |
         echo rver=$(echo ${{ github.event.inputs.rver || 'devel' }}) >> $GITHUB_OUTPUT
-        echo biocver=$(echo ${{ github.event.inputs.biocver || '3.20' }}) >> $GITHUB_OUTPUT
+        echo biocver=$(echo ${{ github.event.inputs.biocver || '3.21' }}) >> $GITHUB_OUTPUT
         echo registryuser=$(echo ${{ github.repository_owner }} | awk '{print tolower($0)}') >> $GITHUB_OUTPUT
         echo rockerintermediateprefix=$(echo "ghcr.io/${{ github.repository_owner }}/rocker" | awk '{print tolower($0)}') >> $GITHUB_OUTPUT
 
@@ -199,7 +199,7 @@ jobs:
       id: defs
       run: |
         echo rver=$(echo ${{ github.event.inputs.rver || 'devel' }}) >> $GITHUB_OUTPUT
-        echo biocver=$(echo ${{ github.event.inputs.biocver || '3.20' }}) >> $GITHUB_OUTPUT
+        echo biocver=$(echo ${{ github.event.inputs.biocver || '3.21' }}) >> $GITHUB_OUTPUT
         echo registryuser=$(echo ${{ github.repository_owner }} | awk '{print tolower($0)}') >> $GITHUB_OUTPUT
         echo rockerintermediateprefix=$(echo "ghcr.io/${{ github.repository_owner }}/rocker" | awk '{print tolower($0)}') >> $GITHUB_OUTPUT
 
@@ -250,7 +250,7 @@ jobs:
       id: defs
       run: |
         echo rver=$(echo ${{ github.event.inputs.rver || 'devel' }}) >> $GITHUB_OUTPUT
-        echo biocver=$(echo ${{ github.event.inputs.biocver || '3.20' }}) >> $GITHUB_OUTPUT
+        echo biocver=$(echo ${{ github.event.inputs.biocver || '3.21' }}) >> $GITHUB_OUTPUT
         echo registryuser=$(echo ${{ github.repository_owner }} | awk '{print tolower($0)}') >> $GITHUB_OUTPUT
         echo rockerintermediateprefix=$(echo "ghcr.io/${{ github.repository_owner }}/rocker" | awk '{print tolower($0)}') >> $GITHUB_OUTPUT
 
diff --git a/.github/workflows/full-rstudio-build.yml b/.github/workflows/full-rstudio-build.yml
index d376eb3..3f891e5 100644
--- a/.github/workflows/full-rstudio-build.yml
+++ b/.github/workflows/full-rstudio-build.yml
@@ -9,7 +9,7 @@ on:
       rver:
         default: "devel"
       biocver:
-        default: "3.20"
+        default: "3.21"
       outname:
         default: "bioconductor_docker"
   schedule:
@@ -61,7 +61,7 @@ jobs:
       run: |
         echo outname=$(echo ${{ github.event.inputs.outname || 'bioconductor_docker' }}) >> $GITHUB_OUTPUT
         echo rver=$(echo ${{ github.event.inputs.rver || 'devel' }}) >> $GITHUB_OUTPUT
-        echo biocver=$(echo ${{ github.event.inputs.biocver || '3.20' }}) >> $GITHUB_OUTPUT
+        echo biocver=$(echo ${{ github.event.inputs.biocver || '3.21' }}) >> $GITHUB_OUTPUT
         echo registryuser=$(echo ${{ github.repository_owner }} | awk '{print tolower($0)}') >> $GITHUB_OUTPUT
         echo rockerintermediateprefix=$(echo "ghcr.io/${{ github.repository_owner }}/rocker" | awk '{print tolower($0)}') >> $GITHUB_OUTPUT
 
diff --git a/Dockerfile b/Dockerfile
index 69b29b2..3e029b2 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -16,7 +16,7 @@ ENV TARGETARCH=${TARGETARCH:-amd64}
 FROM base-$TARGETARCH AS base
 
 ## Set Dockerfile version number
-ARG BIOCONDUCTOR_VERSION=3.20
+ARG BIOCONDUCTOR_VERSION=3.21
 
 ##### IMPORTANT ########
 ## The PATCH version number should be incremented each time
@@ -62,7 +62,7 @@ LABEL name="bioconductor/bioconductor_docker" \
       license="Artistic-2.0"
 
 # Reset args in last layer
-ARG BIOCONDUCTOR_VERSION=3.20
+ARG BIOCONDUCTOR_VERSION=3.21
 ARG BIOCONDUCTOR_PATCH=29
 ARG BIOCONDUCTOR_DOCKER_VERSION=${BIOCONDUCTOR_VERSION}.${BIOCONDUCTOR_PATCH}
 
diff --git a/bioc_scripts/install_bioc_sysdeps.sh b/bioc_scripts/install_bioc_sysdeps.sh
index ffe1e9d..003bc6c 100644
--- a/bioc_scripts/install_bioc_sysdeps.sh
+++ b/bioc_scripts/install_bioc_sysdeps.sh
@@ -1,7 +1,7 @@
 #!/bin/bash
 set -e
 
-BIOC_VERSION=${1:-"3.20"}
+BIOC_VERSION=${1:-"3.21"}
 
 # This is to avoid the error
 # 'debconf: unable to initialize frontend: Dialog'