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DOI Build

Graph construction method impacts variation representation and analyses in a bovine super-pangenome

This work focuses on comparing pangenome constructions methods for the downstream implications of how well (and truthfully) variation is converted into currently-useful formats like VCF.

This reposistory is currently a work-in-progress as code is centralised and cleaned up.

Components

This project involves multiple steps. We first

  • construct pangenomes from 12 bovine assemblies with
    • minigraph
    • pggb
    • cactus
  • decompose pangenomes into vcf
  • compare vcf files for accuracy to assembly-called truth
  • assess applications to new topics like VNTRs.

Citation

The preprint associated with this work can be found here.