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What does cl>49 mean after iter_clust? Because the annotation from Tasic 2016 only covers 0-49 clusters, cl>49 cannot be annotated.
My sample size is around 20k cells. This is the parameter that I used.
de.param <- de_param(padj.th = 0.05,
lfc.th = 1,
low.th = 1,
q1.th = 0.5,
q.diff.th = 0.7,
de.score.th = 150)
Hi,
What does cl>49 mean after iter_clust? Because the annotation from Tasic 2016 only covers 0-49 clusters, cl>49 cannot be annotated.
My sample size is around 20k cells. This is the parameter that I used.
de.param <- de_param(padj.th = 0.05,
lfc.th = 1,
low.th = 1,
q1.th = 0.5,
q.diff.th = 0.7,
de.score.th = 150)
pca.clust.result <- iter_clust(norm.dat,
dim.method = "pca",
de.param = de.param)
Thank you.
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