diff --git a/python/podio/test_Frame.py b/python/podio/test_Frame.py
index aa798a8ae..2d7675215 100644
--- a/python/podio/test_Frame.py
+++ b/python/podio/test_Frame.py
@@ -41,6 +41,7 @@
     "extension_ExternalComponent",
     "extension_ExternalRelation",
     "VectorMemberSubsetColl",
+    "interface_examples",
 }
 
 # The expected parameter names in each frame
diff --git a/tests/read_frame.h b/tests/read_frame.h
index 3a9ec2929..0103afea1 100644
--- a/tests/read_frame.h
+++ b/tests/read_frame.h
@@ -1,6 +1,7 @@
 #ifndef PODIO_TESTS_READ_FRAME_H // NOLINT(llvm-header-guard): folder structure not suitable
 #define PODIO_TESTS_READ_FRAME_H // NOLINT(llvm-header-guard): folder structure not suitable
 
+#include "datamodel/ExampleWithInterfaceRelationCollection.h"
 #include "datamodel/ExampleWithVectorMemberCollection.h"
 #include "read_test.h"
 
@@ -69,6 +70,35 @@ void checkVecMemSubsetColl(const podio::Frame& event) {
   ASSERT(subsetColl[0] == origColl[0], "subset coll does not have the right contents");
 }
 
+void checkInterfaceCollection(const podio::Frame& event) {
+  const auto& interfaceColl = event.get<ExampleWithInterfaceRelationCollection>("interface_examples");
+  ASSERT(interfaceColl.size() == 2, "interface_examples should have two elements");
+
+  const auto& hits = event.get<ExampleHitCollection>("hits");
+  const auto& particles = event.get<ExampleMCCollection>("mcparticles");
+  const auto& clusters = event.get<ExampleClusterCollection>("clusters");
+
+  const auto iface0 = interfaceColl[0];
+  const auto iface1 = interfaceColl[1];
+
+  ASSERT(iface0.aSingleEnergyType() == hits[0], "OneToOneRelation aSingleEnergy not persisted as expected");
+  ASSERT(iface1.aSingleEnergyType() == clusters[0], "OneToOneRelation aSingleEnergy not persisted as expected");
+
+  const auto iface0Rels = iface0.manyEnergies();
+  ASSERT(iface0Rels.size() == 3,
+         "OneToManyRelation to interface does not have the expected number of related elements");
+  ASSERT(iface0Rels[0] == hits[0], "OneToManyRelations to interface not persisted correctly");
+  ASSERT(iface0Rels[1] == clusters[0], "OneToManyRelations to interface not persisted correctly");
+  ASSERT(iface0Rels[2] == particles[0], "OneToManyRelations to interface not persisted correctly");
+
+  const auto iface1Rels = iface1.manyEnergies();
+  ASSERT(iface1Rels.size() == 3,
+         "OneToManyRelation to interface does not have the expected number of related elements");
+  ASSERT(iface1Rels[0] == particles[0], "OneToManyRelations to interface not persisted correctly");
+  ASSERT(iface1Rels[1] == hits[0], "OneToManyRelations to interface not persisted correctly");
+  ASSERT(iface1Rels[2] == clusters[0], "OneToManyRelations to interface not persisted correctly");
+}
+
 template <typename ReaderT>
 int read_frames(const std::string& filename, bool assertBuildVersion = true) {
   auto reader = ReaderT();
@@ -125,6 +155,10 @@ int read_frames(const std::string& filename, bool assertBuildVersion = true) {
     if (reader.currentFileVersion() >= podio::version::Version{0, 16, 99}) {
       checkVecMemSubsetColl(otherFrame);
     }
+    // Interface tests once they are present
+    if (reader.currentFileVersion() >= podio::version::Version{0, 99, 99}) {
+      checkInterfaceCollection(otherFrame);
+    }
   }
 
   if (reader.readNextEntry(podio::Category::Event)) {
diff --git a/tests/write_frame.h b/tests/write_frame.h
index 8a7b71619..1e1c9f966 100644
--- a/tests/write_frame.h
+++ b/tests/write_frame.h
@@ -11,6 +11,7 @@
 #include "datamodel/ExampleWithARelationCollection.h"
 #include "datamodel/ExampleWithArrayCollection.h"
 #include "datamodel/ExampleWithFixedWidthIntegersCollection.h"
+#include "datamodel/ExampleWithInterfaceRelationCollection.h"
 #include "datamodel/ExampleWithNamespaceCollection.h"
 #include "datamodel/ExampleWithOneRelationCollection.h"
 #include "datamodel/ExampleWithVectorMemberCollection.h"
@@ -361,6 +362,24 @@ auto createExtensionExternalRelationCollection(int i, const ExampleHitCollection
   return coll;
 }
 
+auto createExampleWithInterfaceCollection(const ExampleHitCollection& hits, const ExampleClusterCollection& clusters,
+                                          const ExampleMCCollection& particles) {
+  auto coll = ExampleWithInterfaceRelationCollection{};
+  auto elem = coll.create();
+  elem.aSingleEnergyType(hits[0]);
+  elem.addmanyEnergies(hits[0]);
+  elem.addmanyEnergies(clusters[0]);
+  elem.addmanyEnergies(particles[0]);
+
+  elem = coll.create();
+  elem.aSingleEnergyType(clusters[0]);
+  elem.addmanyEnergies(particles[0]);
+  elem.addmanyEnergies(hits[0]);
+  elem.addmanyEnergies(clusters[0]);
+
+  return coll;
+}
+
 podio::Frame makeFrame(int iFrame) {
   podio::Frame frame{};
 
@@ -420,6 +439,8 @@ podio::Frame makeFrame(int iFrame) {
   frame.put(createExtensionExternalComponentCollection(iFrame), "extension_ExternalComponent");
   frame.put(createExtensionExternalRelationCollection(iFrame, hits, clusters), "extension_ExternalRelation");
 
+  frame.put(createExampleWithInterfaceCollection(hits, clusters, mcps), "interface_examples");
+
   return frame;
 }